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KAPA Taq PCR Kits

Overview

The Kapa Taq DNA polymerase enzyme is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. Kapa Taq and Kapa Taq HotStart DNA polymerase enzymes have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated.

Features and Benefits of KAPA Taq PCR Kits

  • Hot-start formulation – combined with a proprietary antibody that inactivates the enzyme until the first denaturation step
  • Elimination of spurious amplification products and increase in reaction efficiency and sensitivity
  • Supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template
  • Also available with loading dye reaction buffer, allowing direct loading of PCR products onto agarose gels with no extra steps for adding loading/tracking dye

Product Highlights

Improved sensitivity, specificity and yield compared to other available kits

  • Novel buffer formulation, facilitating specific primer annealing, leading to higher yield of specific product

View data showing superior PCR performance with the amplification of CCR5 gene fragment using KAPA Taq PCR Kit compared to competitor kits.

See data showing higher sensitivity and less primer dimers using Kapa HotStart enzyme compared to KAPA Taq PCR Kit from mycoplasma DNA fragment amplification.

Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche.