The Kapa Taq DNA polymerase enzyme is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. Kapa Taq and Kapa Taq HotStart DNA polymerase enzymes have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated.
Features and Benefits of KAPA Taq PCR Kits
Hot-start formulation – combined with a proprietary antibody that inactivates the enzyme until the first denaturation step
Elimination of spurious amplification products and increase in reaction efficiency and sensitivity
Supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template
Also available with loading dye reaction buffer, allowing direct loading of PCR products onto agarose gels with no extra steps for adding loading/tracking dye
Product Highlights
Improved sensitivity, specificity and yield compared to other available kits
Novel buffer formulation, facilitating specific primer annealing, leading to higher yield of specific product