Kits may be stored for up to 18 months at -20˚C.

Kits include KAPA Long Range or KAPA Long Range HotStart DNA Polymerase (5 or 2.5 U/μL), MgCl2-free KAPA Long Range Buffer (5X), high-quality dNTP Mix (10 mM each) and MgCl2 (25 mM).  KAPA Long Range HotStart is also available in KAPA Long Range HotStart ReadyMix with dye (2X), which contains DNA Polymerase, reaction buffer with loading dye, dNTPs and MgCl2 (at a final concentration of 2 mM).

Starting Material Genomic DNA, cDNA, plasmid, lambda
Input Amount
< 100 ng for genomic DNA, cDNA < 10 ng for less complex DNA
KAPA Long Range DNA Polymerase (5 U/µL) or KAPA Long Range HotStart DNA Polymerase (2.5 U/µL)
5X KAPA Long Range Buffer (without Mg2+)
MgCl(25 mM)
dNTP Mix (10 mM each dNTP)