Overview

An integrated approach to sample preparation, for applications such as targeted sequencing, encompassing all the steps required to convert a sample to a sequencing-ready library is beneficial in your research considering the precious nature of NGS samples. Therefore, we offer Roche Sample Prep Solutions, from sample collection to library quantification for different sample types and sequencing applications that are proven, simple and complete.

HyperCap products provide an integrated next-generation sequencing (NGS) sample preparation solution that combines all reagents and accessories required for library preparation and target enrichment in a single workflow. The HyperCap Workflow v2.0 integrates high-efficiency KAPA Library Preparation Kits and KAPA Dual-Indexed Adapters with performance-optimized SeqCap® EZ probes and Roche Universal Blocking Oligo (UBO) Kit. The HyperCap Workflow v2.0 is an improved and streamlined version of our SeqCap EZ HyperCap Workflow, with the convenience of ordering and support from a single vendor, and is automation friendly.

KAPA HyperPrep and KAPA HyperPlus Library Preparation Kits are novel one-tube chemistry with optimally formulated enzymes selected through our directed evolution technology. They enable higher yields of adapter-ligated library and lower amplification bias resulting in higher library diversity, lower duplication rates and more uniform coverage compared to other products available in the market.* 

SeqCap EZ probes use an innovative single-tube solution-based hybridization capture technology that enables enrichment of any genomic region of interest. They are built upon an optimized design algorithm and manufactured with high probe density and demonstrate uniform and higher depth of coverage. 

KAPA Dual-Indexed Adapters enable greater multiplexibility compared to existing options by utilizing up to 96 sample barcodes.

Roche Universal Blocking Oligo Kits eliminate the need for adapter-matched blocking oligos, affording a simple and faster workflow. 

 

HyperCap Workflow for Genetic Research

Jennfier Pavlica, Applications Team Manager of Roche Sequencing and Life Sciences, presents Roche Sample Prep Solutions: HyperCap Workflow for Genetic Research

# Step SeqCap EZ v5.1 HyperCap v1.0 HyperCap v2.0
1 Library construction chemistry KAPA HTP/LTP Kit KAPA HyperPrep or HyperPlus Kit KAPA HyperPrep or HyperPlus Kit; SeqCap Adapter A/B Kits, KAPA Dual-Indexed (96) Adapters
2 DNA binding to SPRI beads (cleanups) 15 min 5 min 5 min
3 Hybridization setup Dry-down Bead-based Bead-based; UBO
4 Volume of capture beads 100 µL 50 µL
50 µL
5 Vortex during capture Yes No No
6 Bead capture time 45 min 15 min 15 min
7 Bead wash volume (tube) 200 µL (1.5 mL tube) 200 µL (PCR tube/plate) 200 µL (PCR tube/plate)
8 Bead wash parameters Vortex mixer or water bath needed No vortex or water bath required No prolonged vortexing or water bath required
9 Post-capture LM-PCR 2 x 50 µL reactions, 14 cycles Single 50 µL reaction, 14 cycles Single 50 µL reaction, 14 cycles

Benefits of HyperCap Workflow

Integrated and streamlined workflow

  • Higher efficiency in library preparation process with lower input amounts
  • Higher coverage with low duplicate rates
  • Automation-friendly
  • Ordering and support of all products of the workflow from a single vendor

UBOs allow for easy experiment planning

  • Reduced time and complexity with the elimination of adapter-matched blocking oligo use
  • Flexibility to block 6- and 8-base barcode adapters and use with single- or dual-indexing strategies

Improved performance compared to HyperCap Workflow v1.0

  • Increased percentage coverage and on-target read
  • Reduced total duplicate rate

Efficient library construction with KAPA Dual-
Indexed (96) Adapter Kits 

  • Minimal levels of adapter dimer formation
  • Nominal levels of barcode cross contamination as confirmed by sequencing-based QC testing
  • Higher multiplexibility compared to existing options

Easily automatable workflow with broad platform compatibility 

  • Reduced instrumentation requirement enabling easy automation
  • Multiplexing option, optimizing robot utilization
  • Concordant performance as the manual method

  *Data on file.

Research Use Only. Not for use in diagnostic procedures.
SEQCAP, HYPERCAP and KAPA are trademarks of Roche.