Hybridization-Based Target Enrichment

Overview

In  hybridization or capture-based target enrichment of samples prior to  next-generation sequencing (NGS), pools of biotinylated oligonucleotide probes specific for target regions of interest are hybridized to a sequencing library in solution. Next, the biotinylated probe/target hybrids are pulled down by streptavidin-coated magnetic beads to obtain libraries highly enriched for the target regions.

Roche Sequencing Solutions offers the SeqCap® target enrichment products, which use a solution-based capture method for target enrichment. Find target enrichment options for analyzing the whole exome (SeqCap EZ Human Exome Probes v3 ) or specific disease-associated regions (SeqCap EZ MedExome or customize using SeqCap EZ Prime Choice Probes), for investigating methylation changes (SeqCap Epi) or for studying transcriptomes (SeqCap RNA).

SeqCap® EZ Choice Probes are replaced by SeqCap® EZ Prime Choice Probes, which provide improved GC coverage compared to SeqCap EZ Choice Probes.

Features and Benefits of SeqCap target enrichment products

  • A simple, single-step target enrichment solution based on an optimized design algorithm
  • Various options for the enrichment of the whole exome or regions of  interest, bisulfite-treated DNA or RNA
  • HyperCap Workflow provides an integrated NGS sample preparation solution, combining all reagents and accessories required for library preparation and target enrichment in a single workflow
  • Predesigned catalog options or custom designs based on specific requirements
  • An integrated, application-specific sample preparation solution

Available Products

Below is the list of hybridization-based target enrichment products we offer. 

If you are not sure which target enrichment product would work best for your specific application, use our target enrichment selector tool to fine-tune your selection. You can specify your requirements (for example, nucleic acid, target, size or application) and drill down to the right kit for your need.


SeqCap EZ Probes

Based on a solution-based capture method, the SeqCap EZ Probes enable the enrichment of the whole exome or medically relevant regions of interest. Both catalog and custom (user-defined) designs are available. You can also share or access predesigned custom panels shared by others.

Catalog (pre-designed and off the shelf)
Custom (user-defined)
Shared Design (pre-designed)

SeqCap EZ MedExome Probes

(47 Mb whole exome enrichment design)

SeqCap EZ Prime Choice Probes

(capture up to 7 Mb custom regions)

SeqCap Designs

(Access predesigned custom panels developed by other researchers)

SeqCap EZ Human Exome Probes v3.0

(64 Mb sequence capture design)

SeqCap EZ Prime Choice XL Probes

(capture up to 200 Mb custom regions)

 

SeqCap EZ Exome Plus Probes

(64 Mb exome capture with the ability to add up to 200 Mb of custom targets)

SeqCap EZ Prime Developer Probes

(capture up to 200 Mb custom regions from nonhuman genomes)

 

SeqCap EZ Exome +UTR Probes

(96 Mb design)

   

SeqCap EZ HGSC VCRome Kit

 

   

If you are using SeqCap EZ Choice and SeqCap EZ Choice XL Probes that have not been rebalanced, you can directly substitute with SeqCap EZ Prime Choice Probes. For rebalanced designs, continue to order SeqCap EZ Choice and SeqCap EZ Choice XL Probes.


SeqCap Epi Probes

Built on our proprietary probe design and manufacturing technologies, the SeqCap Epi Target Enrichment Kits enable DNA methylation assessment at single-base resolution by enriching bisulfite-treated DNA. Both catalog and custom designs are available. You can also share or access predesigned custom panels shared by others.

Catalog (pre-designed and off the shelf)
Custom (user-defined)
Shared Designs (pre-designed)

SeqCap Epi CpGiant Probes

(fixed epigenome design to interrogate >5.5 million methylation sites)

SeqCap Epi Choice Probes

(target up to 90 Mb epigenomic regions of interest)

SeqCap Epi Designs
 

SeqCap Epi Developer Probes

(target up to 210 Mb of both strands of any organism’s bisulfite-treated DNA or from 90-200 Mb of human targets)

 

SeqCap RNA Probes

Designed to target the transcripts of interest in your transcriptome, the SeqCap RNA Probes help overcome the challenges in RNA-Seq and transcriptome analyses. Fixed catalog designs to capture long non-coding RNA (lncRNA) are available as well as custom choices with variable design sizes.

Catalog (pre-designed and off the shelf)
Custom (user-defined)

SeqCap lncRNA Probes

(Capture up to 17 Mb of long noncoding RNA)

SeqCap RNA Choice Probes

(target up to 7 Mb to 100 Mb of design size)

 

SeqCap RNA Developer Probes

(target up to 200 Mb in non-human organisms)

Not sure which target enrichment kit would work best for your specific application? Use our target enrichment selector tool to help you choose the right kit for your need.
 

Custom Designs

NimbleDesign, our free online tool, enables you to quickly and easily design custom SeqCap EZ Prime Choice, SeqCap EZ Prime Developer Probes, SeqCap EZ Prime Choice XL or HEAT-Seq® Choice Designs for human and non-human research applications. Learn more about the NimbleDesign software for custom target enrichment designs.

Appropriate reagents and accessories are also available for each of these classes of products.