Amplicon-Based Target Enrichment

Overview

Amplicon-based target enrichment methods for next-generation sequencing (NGS) rely on PCR-amplification of target regions of interest using sequence-specific primers and probes. Using molecular inversion probes (MIP), specific probes consisting of a universal linker flanked by target-specific sequences are constructed. The probes anneal on both sides of the target amplicon and the gap is filled by a DNA polymerase.

Roche Sequencing Solutions offers the HEAT-Seq® portfolio of target enrichment products, which utilize an advanced version of MIP technology, for single nucleotide variation (SNV) detection for targeted sequencing applications in human genetic disease and cancer research. 

Features and benefits of HEAT-Seq portfolio of target enrichment products

  • Fast and easy workflow with powerful SNV detection
  • Reliable performance through the use of an advanced version of MIP
  • Choice between catalog and custom formats  to maximize your time and resources

Roche Sequencing Solutions offers custom HEAT-Seq products

HEAT-Seq Ultra Choice Designs

HEAT-Seq Ultra Choice Designs enable you to build custom designs of varying length (between 32 to 6,000 probes) using a large database containing empirically tested and balanced probes for 60 common oncogenes.

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HEAT-Seq Choice Designs

HEAT-Seq Choice Designs enable you to build custom designs of varying length (between 140 to 27,343 probes) using a large database containing empirically tested and balanced probes for genes associated with human genetic disease.

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HEAT-Seq Oncology Panel

The HEAT-Seq Oncology Panel contains 5,144 probes, and has a capture target of approximately 245 Kb across 60 commonly targeted oncogenes and is ideal for new variant discovery and validation.

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Heat-Seq Ultra Oncology HotSpot Panel

The HEAT-Seq Ultra Oncology HotSpot Panel contains 635 probes, and has a capture target of approximately 30.5 Kb across previously described mutation hotspots in 53 commonly targeted oncogenes and is ideal for detecting and characterizing low-frequency mutations

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HSQutils Software

The HSQutils software is a utility package containing a separately sourced sequencing read mapper and variant caller that is intended for use in an analysis workflow, for which Roche provides periodic updates to this software.




Appropriate reagents and accessories are also available for each of these classes of products.