KAPA SYBR FAST One-Step qRT-PCR Kits

Kits can be stored for up to 6 months at -20˚C.

Kits include KAPA RT Mix, dUTP, and KAPA SYBR FAST qPCR Master Mix, which contains KAPA SYBR FAST DNA Polymerase, reaction buffer, dNTPs, SYBR Green I dye, and MgCl2 at a final concentration of 2.5 mM. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes 50X ROX High and 50X ROX Low separately.

Please reference the Instrument Compatibility Chart for guidance on compatible platforms.

Specifications
Spec
Description
Starting Material
RNA
Input Amount 1 pg–1 µg total RNA, 10 fg–100 ng poly(A)RNA,  10-1x108 copies viral RNA

Components
   Kit code  50X KAPA SYBR FAST ROX High 50X KAPA SYBR FAST ROX Low 2X KAPA SYBR FAST qPCR Master Mix (Universal) 2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye) 50X KAPA RT Enzyme Mix 10mM dUTP
Universal

KK4650

KK4651

KK4652

X X X   X X
ABI Prism

KK4660

KK4661

KK4662

      X X X
Bio-Rad iCycler

KK4670

KK4671

KK4672

      X X X
Optimized for Roche LightCycler® 480

KK4680

KK4681

KK4682

      X X X