KAPA SYBR FAST One-Step qRT-PCR Kits

Overview

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kits contain an M-MuLV reverse transcriptase, an RNase inhibitor, and a novel DNA polymerase engineered through our directed evolution technology.

Benefits of KAPA SYBR FAST One-Step qRT-PCR Kits

  • Rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination
  • A convenient qRT-PCR protocol

Product Highlights

Increase signal and reaction efficiency

  • Consistently higher amplification efficiencies *
  • Higher fluorescence, earlier Cq  values and improved reaction efficiencies compared to other options*

Amplify across a broad range of target lengths

  • Robust performance independent of amplicon size

Improve sensitivity and reproducibility

  • Accurate interrogation across a wide range of RNA template concentrations

*Data on file.