Overview

The Kapa Taq DNA polymerase enzyme is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. Kapa Taq and Kapa Taq HotStart DNA polymerase enzymes have 5′-3′ polymerase and 5′-3′ exonuclease activities, but no 3′ to 5′ exonuclease (proofreading) activity. The enzyme has an error rate of approximately 1 error per 2.2 x 105 nucleotides incorporated.

Features and Benefits of KAPA Taq PCR Kits

  • Hot-start formulation – combined with a proprietary antibody that inactivates the enzyme until the first denaturation step
  • Elimination of spurious amplification products and increase in reaction efficiency and sensitivity
  • Supplied in a 2X ReadyMix format containing all the components required for PCR except primers and template
  • Also available with loading dye reaction buffer, allowing direct loading of PCR products onto agarose gels with no extra steps for adding loading/tracking dye

Product Highlights

Improved sensitivity, specificity and yield compared to other available kits

  • Novel buffer formulation, facilitating specific primer annealing, leading to higher yield of specific product