Overview

The KAPA RNA HyperPrep Kits utilize a novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality RNA-seq libraries. The strand-specific workflow is flexible, supporting library construction from lower-input amounts and degraded samples and is compatible with mRNA capture and ribosomal RNA and/or globin transcript depletion. 

Every KAPA RNA HyperPrep Kit includes the same core library construction chemistry with an optional upfront depletion module, making up four workflow solutions: either mRNA capture, rRNA depletion, rRNA and globin depletion or none. Roche Sequencing Solutions provides a complete library preparation solution with KAPA Pure Beads (included) and KAPA Dual-Indexed Adapters (available separately). These kits are designed to be automation-friendly on a majority of commercially available liquid handlers.

 

 

Features and Benefits of KAPA RNA HyperPrep Kits

  • Reduced hands-on and overall time through fewer enzymatic and reaction cleanup steps* 
  • Strand-specific, sequencing-ready libraries from input RNA in ~4 hours*
  • Single-day library construction, including mRNA capture and rRNA depletion with the KAPA RNA HyperPrep workflow*
  • Robust performance across different sample types and input amounts*
  • High throughput and consistency with an automation-friendly workflow
  • Validated KAPA RNA HyperPrep Kit data analysis solutions provided through the Genialis platform 

Product Highlights

Flexible workflow options for a variety of applications

  • Stranded library construction solutions for sequencing of both coding and non-coding transcripts
  • Compatible with a variety of sample types and input amounts, of both high-quality and degraded RNA

Single-day, single-tube library prep
  • Reduce hands-on and overall turnaround time with fewer enzymatic and cleanup steps
  • Produce strand-specific libraries from input RNA in approximately 4 hours
  • Complete the entire workflow, inclusive of upfront RNA enrichment, in a standard work day
  • Achieve high throughput and consistency with automation-friendly workflows

Sequence what matters

  • Effective RNA enrichment (rRNA depletion or mRNA capture), combined with highly efficient library construction, reduces the number of reads associated with unwanted content and PCR duplicates
  • More unique transcripts and genes are detected from a fixed amount of sequencing

Cover your bases with higher uniformity

  • Efficient RNA enrichment and library construction processes results in more even coverage along transcript lengths
  • Library amplification with KAPA HiFi enables better coverage of difficult GC-rich region

Streamlined end-to-end RNA library preparation and analysis workflow

  • By partnering with Genialis to develop an automated, data analysis and visualization solution validated for KAPA RNA HyperPrep Kits, Roche Sequencing Solutions provides customers with an end-to-end RNA workflow that is simple, proven and complete.
  • Allows every processing step to be traced to ensure quality and reproducibility and management of data, projects and collaborations in the cloud. 

 

*Data on file.