The KAPA RNA HyperPrep Kits utilize a novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality RNA-seq libraries. The strand-specific workflow is flexible, supporting library construction from lower-input amounts and degraded samples and is compatible with mRNA capture and ribosomal RNA and/or globin transcript depletion.
Every KAPA RNA HyperPrep Kit includes the same core library construction chemistry with an optional upfront depletion module, making up four workflow solutions: either mRNA capture, rRNA depletion, rRNA and globin depletion or none. Roche Sequencing Solutions provides a complete library preparation solution with KAPA Pure Beads (included) and KAPA Dual-Indexed Adapters (available separately). These kits are designed to be automation-friendly on a majority of commercially available liquid handlers.
Features and Benefits of KAPA RNA HyperPrep Kits
Reduced hands-on and overall time through fewer enzymatic and reaction cleanup steps*
Strand-specific, sequencing-ready libraries from input RNA in ~4 hours*
Single-day library construction, including mRNA capture and rRNA depletion with the KAPA RNA HyperPrep workflow*
Robust performance across different sample types and input amounts*
High throughput and consistency with an automation-friendly workflow
Validated KAPA RNA HyperPrep Kit data analysis solutions provided through the Genialis platform
Streamlined end-to-end RNA library preparation and analysis workflow
By partnering with Genialis to develop an automated, data analysis and visualization solution validated for KAPA RNA HyperPrep Kits, Roche Sequencing Solutions provides customers with an end-to-end RNA workflow that is simple, proven and complete.
Allows every processing step to be traced to ensure quality and reproducibility and management of data, projects and collaborations in the cloud.