KAPA Library Quantification Kits

Kits can be stored at -20˚C.

Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.

Specifications
Spec Description
Compatible Platforms Illumina HiSeq, NextSeq, MiSeq, and GAIIx Ion Torrent Proton and PGM 454 GS FLX+ and GS Junior SOLiD 5500 series
Library Type
Any
Starting Material
Any NGS library ready for cluster amplification or emPCR
Standard curve concentration range
20–0.0002 pM for Illumina libraries 83–0.00083 pM for Ion Torrent and 454 libraries 10–0.0001 pg/µL for SOLiD libraries
Sequencing Applications
Whole Genome Sequencing, Whole Exome Sequencing, Targeted Sequencing (custom panels), RNA-Seq, ChIP-Seq, Amplicon Sequencing, Methyl-Seq
Components–Illumina Platform
  Kit code

10X Illumina

Primer Premix

2X KAPA  

SYBR FAST qPCR

Master Mix

(universal)

50X KAPA

SYBR FAST ROX High

50X KAPA

SYBR FAST ROX Low

2X KAPA

SYBR FAST qPCR

Master Mix

(with equipment-specific dye)

2X KAPA

SYBR FAST

qPCR Master Mix

(ROX Low)

Illumina DNA Standards 1-6
Complete Kit (universal)
KK4824 X X X X     X
Complete Kit (with equipment-specific dye)

KK4835

KK4844

KK4854

X       X   X
Complete Kit (ROX Low)

KK4873

 

X         X X
qPCR MasterMix (universal) and Primer Premix only KK4923 X X X X      

qPCR MasterMix (with equipment-specific  reference dye)

 and Primer Premix only

KK4933

KK4943

KK4953

X       X    
qPCR MasterMix (ROX Low)
and Primer Premix only
KK4973 X         X  
Components–Ion Torrent Platform
   Kit Code 

10X Illumina

Primer Premix

2X KAPA  

SYBR FAST qPCR

Master Mix

(universal)

50X KAPA

SYBR FAST ROX High

50X KAPA

SYBR FAST ROX Low

2X KAPA

SYBR FAST qPCR

Master Mix

(with equipment-specific dye)

2X KAPA

SYBR FAST

qPCR Master Mix

(ROX Low)

Illumina DNA Standards 1-6
Complete Kit (universal)
KK4827 X vX X X     X
Complete Kitq (with equipment-specific dye)

KK4838

KK4847

KK4857

 

X

     

 

X

 

 

X

Complete Kit (ROX Low)

KK4874

 

 

X

       

 

X

 

X

qPCR MasterMix (universal) and Primer Premix only KK4924 X X X X      

qPCR MasterMix (with equipment-specific  reference dye) 

and Primer Premix only

KK4934

KK4944

KK4954

 

X

     

 

X

   
qPCR MasterMix (ROX Low)
and Primer Premix only
KK4974 X
        X