Library preparation for next generation sequencing (NGS) involves several key steps: fragmentation of nucleic acids to required lengths, addition of platform-specific adapters, optional library amplification, and library quantification and/or normalization. In both DNA and RNA (cDNA) library construction workflows, it is often necessary to select for fragments of the appropriate size. Removing unused adapters, primers and dimers is critical prior to sequencing. Adapter and bead quality and performance can have a profound impact on the outcome of library construction and sequencing, particularly when working with low-input and challenging samples.
Roche Sequencing Solutions offers high-quality adapters and beads that are functionally tested in NGS library construction workflows, and meet strict QC specifications. KAPA Adapters and KAPA Pure Beads are available as stand-alone products to offer you maximum flexibility.