Overview

KAPA HiFi Uracil+ DNA Polymerase is a modified version of KAPA HiFi DNA Polymerase that is engineered to tolerate uracil residues in bisulfite-treated DNA. Bisulfite treatment of DNA results in the conversion of unmethylated cytosine residues into uracil, while the methylated residues are left unmodified. Enzymes used for library amplification in bisulfite-sequencing workflows must be able to read through uracil residues and tolerate low concentrations of AT-rich DNA. Traditional high-fidelity DNA polymerases are typically not suitable for this type of application as the enzymes stall when a uracil is encountered. KAPA HiFi Uracil+ DNA Polymerase has been developed to read through uracil residues while still retaining the performance benefits of HiFi DNA Polymerase, such as high yields, low-bias, and uniform sequencing coverage.

Benefits of KAPA HiFi Uracil+ Kit

  •  Higher yields and reduced size bias for templates containing  uracil compared to other methods
  •  More uniform bisulfite-sequencing coverage and improved  representation of AT-rich sequences compared to competitor kits

Product Highlights

Amplification of uracil-containing template

  • Effective amplification through uracil residues

High yields of low-bias bisulfite-converted library generation

  • Reduced cycles and lower PCR duplicates through high-efficiency amplification
  •  Improved representation of all library fragments and sequence regions due to low amplification bias

Improved representation of AT-rich sequences

  • Greater tolerance to AT-rich regions
  • Improved bisulfite-sequencing read quality