KAPA HTP/LTP Library Preparation Kits

KAPA HTP/LTP Library Preparation Kits contain optimally formulated enzymes selected through our directed evolution technology and implement a highly optimized “with bead” strategy that enable higher library construction efficiency and facilitate automation.  Kits offer higher success rates than conventional library construction kits — particularly from lower inputs and challenging samples such as FFPE and ChIP DNA —  in both high- and low-throughput sample preparation pipelines. KAPA HTP/LTP Library Preparation Kits provide a complete library preparation solution with KAPA Adapters and KAPA Pure Beads.

Features and Benefits of KAPA HTP/LTP Library Preparation Kits

• Automation-friendly, highly optimized “with-bead” protocol facilitating minimal sample loss with no physical transfer of library material between enzymatic reactions

• Improved coverage uniformity as a result of low-bias library amplification with KAPA HiFi enzyme 

• Compatibility with a wide range of DNA sample types, inputs and sequencing applications, and flexibility with respect to fragment size, adapter design, and library amplification 

• Qualified automation methods

 

Product Highlights

Greater molecular complexity

  • Higher adapter-ligated library yields, translating to higher library complexity
  • Fewer cycles of amplification needed, resulting in lower PCR duplication rates, which are critical for library construction from lower input and challenging samples

Reduced amplification bias and improved coverage

  • Reduced PCR bias* using KAPA HiFi enzyme
  • Improved coverage uniformity of across the entire range of genomic GC content













Automation with qualified scripts

  • Qualified methods available for major liquid handling platforms used in NGS, such as PerkinElmer Sciclone NGS and NGSx, Beckman Coulter Biomek FXp and FX dual-pod, Agilent Technologies NGS Workstation Option B, and Eppendorf® epMotion 5075 TMX

*Data on file.