Automation Support Program

Overview

Optimizing NGS sample preparation steps for automation

Roche Sequencing Solutions allows you to unlock the potential of every sample by providing automation scripts and support for our Sample Prep Reagents & Workflows across a wide offering of 3rd-party NGS liquid handling platforms.

Workflows supporting KAPA DNA and RNA Library Preparation Kits, KAPA Library Quantification Kits and Roche Target Enrichment (HyperCap Workflow & SeqCap EZ Probes) are validated and optimized for automated, high-throughput NGS library construction. Scripts are currently available for many NGS liquid handling platforms and are continuously being developed to accommodate other Roche Sample Prep Reagents, Workflows and liquid handling platforms. In addition, the necessary overage volumes in our 96-reaction kits enable automation-friendly workflows to enable your research.

Features 

  • Validated and supported automation scripts and workflows from Roche and 3rd-party vendors 
  • Streamlined and optimized workflows for high performance KAPA DNA or RNA Library Preparation Kits and the HyperCap Workflow using SeqCap EZ Probes
  • Necessary overage volumes in 96-reaction kits to enable automation-friendly workflows


Benefits 

  • Higher sample throughput
  • Less hand-on time for greater convenience of batch processing up to 96 samples 
  • Greater reproducibility than manual
  • Improved process control 
  • Comparable performance with manual methods


Automation Methods Availability

The following tables showcase the vendors with which we have partnered to create automation methods. Search the table below by vendor or by product to find information regarding your specific needs. Please contact your local Roche representative for more information regarding our automation methods and for information on products not listed below.

Availability may differ by region. Please contact your local Roche Sales Representative to learn more.

PerkinElmer

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • HyperCap Workflow
  • SeqCap EZ Probes
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)
  • KAPA mRNA HyperPrep Kit

 

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • KAPA RNA HyperPrep Kit
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)
  • KAPA mRNA HyperPrep Kit
  • HyperCap Workflow*


*in development

Method Source: PerkinElmer

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit

Beckman Coulter

Method Source: Roche

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit*
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit*
  • HyperCap Workflow
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)*
  • KAPA mRNA HyperPrep Kit*


*in development

  Method Source: Beckman Coulter

  • KAPA Library Quantification Kit

  Method Source: Roche

  • KAPA Library Quantification Kit*


*in development

Method Source: Beckman Coulter

  • KAPA HyperPrep Kit*
  • KAPA HyperPlus Kit*

 

*in development

Method Source: Roche

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • HyperCap Workflow*

 

*in development

Agilent

Method Source: Agilent

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • SeqCap EZ Probes


Method Source: Roche

  • KAPA Library Quantification Kit*


*in development

Method Source: Roche

  • KAPA HyperPrep Kit*
  • KAPA HyperPlus Kit*
  • KAPA Library Quantification Kit
  • KAPA Human Genomic DNA Quantification and QC Kit
  • Library Normalization*
  • HyperCap Workflow


Method Source: Agilent

  • SeqCap EZ Probes
  • KAPA mRNA HyperPrep Kit

 

Eppendorf

Method Source: Eppendorf

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • KAPA RNA HyperPrep Kit*
  • KAPA mRNA HyperPrep Kit*


*in development

 

Hamilton

Method Source: Hamilton

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit
  • HyperCap Workflow*
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)*
  • KAPA mRNA HyperPrep Kit*


*in development

 

Tecan

Method Source: Tecan

  • KAPA HyperPrep Kit
  • KAPA HyperPlus Kit
  • KAPA Library Quantification Kit*
  • SeqCap EZ Probes
  • KAPA RNA HyperPrep Kit*
  • KAPA RNA HyperPrep Kit with RiboErase (HMR)*
  • KAPA mRNA HyperPrep Kit*


*in development

 

PrimaDiag

Method Source: PrimaDiag

  • KAPA HyperPlus Kit
  • SeqCap EZ Probes

 

 

Brand

Method Source: Brand

  • KAPA Library Quantification Kit*

 

*in development

 

 

KAPA HyperPrep Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

 

Method Source: Beckman Coulter

  • Biomek i5 Automated Workstation*

 

*in development

Method Source: Agilent

  • Bravo NGS Workstation Option A


Method Source: Roche

  • Bravo NGS Workstation Option B

 

 

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (8 channel)

Method Source: Tecan

  • Freedom Evo NGS Workstation

KAPA HyperPlus Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation

 

Method Source: Beckman Coulter

  • Biomek i5 Automated Workstation*

 

*in development

Method Source: Agilent

  • Bravo NGS Workstation Option A


Method Source: Roche

  • Bravo NGS Workstation Option B



Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (8 channel)

Method Source: Tecan

  • Freedom Evo NGS Workstation

Method Source: PrimaDiag

  • ACSIA NGS (capture)

KAPA Library Quantification Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek 4000*


Method Source: Beckman Coulter

  • Biomek NXp
  • Biomek NXp (Span 8)


*in development

Method Source: Roche

  • Bravo NGS Workstation Option A*
  • Bravo NGS Workstation Option B


*in development

Method Source: Eppendorf

  • epMotion 5075 TMX

Method Source: Hamilton

  • NGS STAR (8 channel)

Method Source: Tecan

  • Freedom Evo NGS Workstation*


*in development

Method Source: Brand

  • Liquid Handling Station*


*in development

KAPA Human Genomic DNA Quantification and QC Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3R NGS Express



Method Source: Roche

  • Biomek FXp and FX, dual pod*


*in development

Method Source: Roche

  • Bravo NGS Workstation Option B*


*in development

Library Normalization

Method Source: Roche

  • Bravo NGS Workstation Option B*


*in development

HyperCap Workflow

Method Source: PerkinElmer

  • Sciclone G3 NGSx

Method Source: Roche

  • Biomek FXp and FX, dual pod
  • Biomek i7 Automated Workstation*

 

*in development

Method Source: Roche

  • Bravo NGS Workstation Option B

Method Source: Hamilton

  • NGS STAR (8 channel)*

*in development

*in development
*in development

SeqCap EZ Workflow

Method Source: PerkinElmer

  • Sciclone G3 NGSx

Method Source: Agilent

  • Bravo NGS Workstation Option A
  • Bravo NGS Workstation Option B

Method Source: Tecan

  • Freedom Evo NGS Workstation

Method Source: PrimaDiag

  • ACSIA NGS (capture)

KAPA RNA HyperPrep Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS

Method Source: Roche

  • Biomek FXp and FX, dual pod*


*in development

Method Source: Eppendorf

  • epMotion 5075 TMX*


*in development

Method Source: Hamilton

  • NGS STAR (8 channel)*


*in development

Method Source: Tecan

  • Freedom Evo NGS Workstation*


*in development

KAPA RNA HyperPrep Kit with RiboErase (HMR)

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3 R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod*


*in development

Method Source: Hamilton

  • NGS STAR (8 channel)*


*in development

Method Source: Tecan

  • Freedom Evo NGS Workstation*


*in development

KAPA mRNA HyperPrep Kit

Method Source: PerkinElmer

  • Sciclone G3 NGSx
  • Zephyr G3 NGS
  • JANUS G3 R NGS Express

Method Source: Roche

  • Biomek FXp and FX, dual pod*


*in development

Method Source: Agilent

  • Bravo NGS Workstation Option B

 

Method Source: Eppendorf

  • epMotion 5075 TMX*


*in development

Method Source: Hamilton

  • NGS STAR (8 channel)*


*in development

Method Source: Tecan

  • Freedom Evo NGS Workstation*


*in development