Human Whole-Genome Sequencing in a New Era Defined by the Illumina® HiSeq X® and NovaSeq™ Platforms

24 OCTOBER 2017 24 OCTOBER 2017

In case you missed it, our webinar “Human whole-genome sequencing in the HiSeq X and NovaSeq era” is now available online.

Illumina’s HiSeq X® and NovaSeq™ series of sequencers have ushered in a new era of high-throughput human whole-genome sequencing. Advances in sequencing technology continually drive the evolution of sample preparation, sequencing and primary data analysis processes in the pursuit of robust, production-scale pipelines.

In this webinar sponsored by Roche, each of the presenters provided an overview of their large-scale WGS library and sequencing pipelines, and discussed the optimization they have done for sequencing on the HiSeq X platform. They also shared their experiences with the NovaSeq platform to date. Other current topics, such as sequencing of FFPE samples, a comparison of clinical exome vs. whole-genome data, and sequencing of large, non-human genomes were also addressed.

 

Speaker Biographies


Robert Fulton, M.S.
Director of Development
McDonnell Genome Institute at Washington University, St. Louis, MO, US

Robert (Bob) Fulton is the Director of Development at the McDonnell Genome Institute as Washington University (MGI), where he is responsible for delivering genomic solutions to a broad range of clinical and research questions. Bob joined MGI in 1994, and has over 23 years of experience in the generation and analysis of DNA sequence. Throughout his time at MGI, he has led production-scale targeted sequencing efforts, the sequence improvement (finishing) pipelines responsible for genome sequence refinement; and most recently, project and technical development at MGI. The skills and expertise he has developed over the years are now applied to the development of sequence-based strategies designed to answer critical questions associated with the use of genomics in the clinical and research environments.

 


HarshaVardhan Doddapaneni, Ph.D.
Assistant Professor
Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA

Dr. Doddapaneni has a broad background in molecular biology and genomics, with specific training and expertise in key research areas of NGS sample preparation and management of large-scale sample preparation operations. Since joining the BCM-HGSC in 2012, he has directed the front-end procedures of sample intake, DNA and RNA library construction for next-gen platforms and exome capture. He built automated library preparation pipelines for HiSeq and NovaSeq platforms, and developed multiple protocols for DNA and RNA sequencing from challenging samples. He also has considerable experience in evaluation and application of early access protocols and emerging technologies and provides technical support to the HGSC-Clinical Laboratory

Research Use Only. Not for use in diagnostic procedures.

This XTalks webinar was in part sponsored by Roche Sequencing Solutions. Dr Doddapaneni and Mr. Fulton received a small stipend for their participation.

HISEQ and NOVASEQ are trademarks of Illumina, Inc.