16 OCTOBER 2018 20 OCTOBER 2018

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Meet Roche at ASHG: CoLabs on Overcoming the Challenges of Ultra-Low Input RNA for Single-Cell RNA Sequencing, and NGS-based CRISPR QC 

We are excited to be part of ASHG 2018! We have a jam-packed schedule – from coLabs to demos to announcing an exciting new collaboration. Stop by Booth 715 to find out more about our integrated NGS workflows, or how our new collaboration may simplify your gene expression analysis projects. Make sure you get your own Smithers – a USB drive fully loaded with videos and white papers to help move your research forward! 

If you are interested in single-cell RNA sequencing and/or CRISPR QC, join us for our colabs located in booth 1441 and listen to our experts present data and offer valuable tips on these topics:

Robust library preparation for improved ultra-low input and flexible single-cell RNA sequencing

Time: Wednesday, October 17: 4:00 PM - 4:30 PM; booth 1441 
Speaker: Nancy Nabilsi, Senior Application Scientist, Roche

As the impact of cellular heterogeneity on biological processes continues to be illuminated in biomedical research, there is great interest in molecular profiling of specific cell sub-populations, sometimes down to single-cell resolution. Ultra-low input and/or single-cell RNA-sequencing is a widely adopted method for transcriptome profiling, but the inherently low input quantities of RNA obtained from restricted cell populations pose a challenge to successful NGS library preparation. In this session, we will present data showing high-quality performance from RNA-seq libraries prepared from as little as 1 ng of input RNA using the KAPA RNA HyperPrep workflow combined with mRNA-capture and ribosomal depletion technologies. Additionally, data will be shown highlighting the use of the KAPA HyperPlus workflow with amplified cDNA inputs for more flexible and robust sample processing in single-cell RNA-seq applications.

Enabling NGS-based CRISPR QC High applications with robust high-performance DNA library preparation workflows

Time: Friday, October 19: 3:15 PM - 3:45 PM; booth 1441
Speaker: Jason Liu, Field Application Scientist, Roche

Discovery of the CRISPR system has enabled simple and cost-effective gene editing with significant application potential in biomedical research. Many gene editing experiments have an obligatory QC step involved, either for DNA sequence verification at the targeted genomic locus, or for identification of off-target mutations. Rapid adoption of CRISPR technology coupled with applications across multiple model systems necessitates quick, accurate, and cost-effective sequencing of edited cell lines and/or organisms. In this session, we will present two methodologies leveraging the KAPA DNA library prep portfolio for sequencing-based CRISPR quality control (QC): a high-throughput targeted amplicon approach for indel screening, and CIRCLE-Seq for off-target effect detection.


Research Use Only. Not for use in diagnostic procedures.

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