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Roche Webinar Series
KAPA RiboDesigner: A simplified custom probe design solution for rRNA depletion
Wednesday, November 18th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Ranjit Kumar, Ph.D. Sr. Bioinformatics Scientist, Roche Sequencing and Life Science
Ribosomal RNA (rRNA) accounts for majority of total cellular RNA, and efficient depletion of rRNA is an important step in RNA-sequencing (RNA-seq) for good transcript coverage and cost effectiveness. KAPA RiboDesigner is Roche’s latest custom probe design solution that helps the KAPA RNA HyperPrep RiboErase users to easily design custom probes for studying the transcriptomics of multiple species without going through the complex, time-consuming, and costly manual probe design process.
In this webinar, we will discuss:
- Ribosomal RNA and the challenges it brings to RNA-seq
- Bioinformatics challenges in ribosomal depletion
- KAPA RNA HyperPrep RiboErase Workflow
- KAPA RiboDesigner custom probe design process
- Evaluation of E. coli using KAPA RiboDesigner
- Evaluation of mixed bacterial species using KAPA RiboDesigner
Overcome the challenges of RNA-seq using KAPA RNA HyperPrep
Tuesday, November 3th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Drew Cheney, Field Application Specialist, Roche Sequencing and Life Science
RNA-sequencing (RNA-seq) and the study of transcriptomes have become essential in both molecular and clinical research. Many researchers and sequencing service providers have found RNA-seq difficult to adopt due to various challenges.
In this webinar, we discussed:
- What RNA-seq is and why it is crucial in research
- Unique RNA-seq challenges in sample handling, library preparation, and data analysis
- KAPA RNA HyperPrep workflows and how they can help overcome these challenges
- Q&A with an experienced Roche field application specialist
Investing in the right sample prep solutions to maximize your sequencing resources
Wednesday, October 14th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenters:
Jonathan Nowacki, Senior Technical Services Consultant - Bioinformatics, Roche Sequencing and Life Science;
The right set of NGS tools can save your business— or your lab— valuable sequencing resources. To maximize efficiency and effectiveness, ask yourself: (1) Which approach is best for answering specific experimental questions? (2) Is this the most cost-effective method to get the job done?
In this webinar, we will:
- The economics of sequencing; Where are your resources going?
- GC bias; How does GC bias impact the value?
- Analysis of dropouts (zero coverage) and low-coverage genes; How do these impact value?
- A customizable metric for analyzing value, enabling you to gain insights to improve efficiency
New KAPA Target Enrichment: Better by Design
Wednesday, September 2nd 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Drew Cheney, Field Application Specialist, Roche Sequencing and Life Science
Building on over 10 years of design expertise, Roche is pleased to introduce the KAPA Target Enrichment Portfolio. These new probes for hybridization-based target enrichment are available in the new KAPA HyperExome or in Custom Panels that you develop using HyperDesign , our new online development tool.
In this webinar, we:
- Shared the improved benefits offered by KAPA Target Enrichment probes, especially when paired with our KAPA library prep workflows
- Compared the performance and efficiency of KAPA Target Enrichment probes and the HyperCap Workflow v3 to other widely used targeted enrichment products
- Introduced HyperDesign, our new probe design interface that provides customized probe selection, and live expert assistance at your fingertips
AVENIO Millisect System: Optimize Tumor Yield with Precise Dissection
Wednesday, September 16th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenters:
Teri Rambo Mueller, Field Applications Consultant, Roche Sequencing and Life Science;
Daniel Kim, Instrument Sales Specialist, Roche Sequencing and Life Science
In the age of precision medicine, molecular testing by next-generation sequencing and PCR are transforming oncology decision making. The clinical value of that molecular testing is significantly enhanced by precise dissection of formalin-fixed paraffin-embedded tissue samples.
The AVENIO Millisect System is an automated, high-performance FFPE tissue-dissection system that enables the precise and consistent recovery of tissue areas of interest for molecular pathology. Easily integrated into a variety of clinical workflow configurations, the AVENIO Millisect System can help labs efficiently extract more clinically relevant information from every sample. Accurate dissection improves the results of diagnostic testing by reducing false negatives and allowing identification of additional potential therapeutic targets.
In this webinar, we:
- Introduced the AVENIO Millisect System
- Provided a demonstration of the tissue dissection process in action
RNA-Seq Applications for SARS-CoV-2 Research
Wednesday, August 26th 2020, 1:00 p.m. ET, 10 a.m. PT
Presenter: Dr. Sarah Trusiak, PhD, Senior Applications Scientist Roche Sequencing and Life Science
Since the emergence of the SARS-CoV-2 virus in late 2019, many research groups have pivoted their focus to studying the virus and its associated disease, COVID-19. RNA-seq offers a powerful, high-throughput tool for studying both the genome of RNA viruses, such as SARS-CoV-2, and the resulting transcriptional changes in a host during viral infection.
In this webinar, we:
- Presented several applications of RNA-seq in viral research, including host response, drug discovery, and viral genome mutation analysis, while highlighting examples from recent preprints and publications
- Discussed specific RNA-seq workflows for each viral application