KAPA HyperPETE: The Performance of hybrid-capture TE with the speed & simplicity of amplicon workflows

Presenter: Dustin Masser, PhD, Roche Sequencing & Life Science

KAPA HyperPETE is a novel target enrichment technology that is designed to combine the performance of hybrid-capture with the ease-of-use and turnaround time of amplicon-based workflows. In addition, it is optimized to detect all major somatic variant types (SNV, CNV, InDel, MSI status, and novel fusion transcript partners), and can be performed on tissue DNA, plasma DNA, and tissue RNA samples.

In this webinar, we reviewed:

• The science behind Primer Extension Target Enrichment (PETE) technology
• How KAPA HyperPETE reduces bias and increases the accuracy of sequencing data
• Performance metrics for KAPA HyperPETE panels for somatic tissue DNA, somatic tissue RNA, and cfDNA inputs

NGS Target Enrichment Methods, Performance Metrics, and Application Data

Presenter: Speaker: Brian Godwin, Director of Reagent and Assay Development, Roche Sequencing Solutions

What is Primer Extension Target Enrichment (PETE)?

In this short 10min video you will learn how this newly developed technology uses DNA polymerase extension reactions to provide a high-performance target enrichment solution that is easy to use and can be performed in a single day.