KAPA SARS-CoV-2 panel achieves more uniform coverage compared to the ARTIC amplicon method. IGV coverage plot of one representative replicate of the KAPA SARS-CoV-2 TE dataset (green) compared to a replicate from the ARTIC workflow with v3 primers (gray). A near dropout of reads (black box) was observed in the Spike gene for the ARTIC libraries in all 3 replicates tested while the KAPA SARS-CoV-2 TE yielded more even coverage across the whole Spike region.
The KAPA SARS-CoV-2 Target Enrichment Panel identifies the mutations associated with 6 variants in a single mixed sample. A total of 1,000,000 SARS-CoV-2 genome copies from 6 different variants was combined with either 20 ng (dark blue) or 100 ng (yellow) of human RNA and processed in triplicate using the KAPA RNA HyperPrep Kit and the KAPA SARS-CoV-2 Target Enrichment Panel with the standard 16-hour hybridization. Alternate allele frequency analysis shows that all expected mutations from the six SARS-CoV-2 variants were identified at close to the expected frequency. Datasets downsampled to 1 million reads prior to analysis.