SeqCap EZ HyperCap Workflow


The SeqCap® EZ HyperCap Workflow provides an integrated workflow solution that combines high-efficiency KAPA Library Preparation Kits with performance-optimized SeqCap capture protocol. It is an application-specific approach for efficient, streamlined and automatable targeted sequencing.

KAPA HyperPrep and KAPA HyperPlus Library Preparation Kits are novel one-tube chemistry with optimally formulated enzymes selected through our directed evolution technology. They enable higher yields of adapter-ligated library and lower amplification bias resulting in higher library diversity, lower duplication rates and more uniform coverage compared to other products available in the market.* 

SeqCap EZ target enrichment products use an innovative single-tube solution-based hybridization capture technology that enables enrichment of any genomic region of interest. Built upon an optimized design algorithm and manufactured with high probe density, the SeqCap EZ target enrichment products demonstrate uniform and higher depth of coverage, enabling discovery and detection of variants. 

The SeqCap EZ HyperCap Workflow

Overview of SeqCap EZ HyperCap Workflow for Target Enrichment. The SeqCap EZ HyperCap Workflow has been designed with improvements throughout the library prep and target enrichment process.

Workflow of the SeqCap EZ protocols. By implementing the SeqCap EZ HyperCap protocol, the user can reduce the workflow time into 1.5 days - from extracted DNA to sequencing ready library.

Benefits of SeqCap EZ HyperCap Workflow

Speed with streamlined workflow

  • Hybridization-based capture with enzymatic or mechanical-shearing protocol
  • Greatly reduced DNA fragmentation and library construction time in comparison to commonly used library preparation protocols*  

Easily automatable workflow with broad platform compatibility 

  • Reduced instrumentation requirement enabling easy automation
  • Workflow allows multiplexing, thus optimizing robot utilization

Concordant performance as the manual method

Automation compatibility of SeqCap Ez HyperCap workflow

Automation platforms available for use with SeqCapEZ enrichment products.

Note: The availability of “Standard Solutions” is constantly changing. Contact your instrument providers or see our automation page to obtain the most up-to-date list of available applications. 


*Data on file.