1. Roche Sequencing
  2. PCR
  3. KAPA2G Fast PCR Kits
  4. Specifications

KAPA2G Fast PCR Kits

Kits can be stored for up to 18 months at -20˚C.

Standard kits include KAPA2G Fast (5 U/μL), KAPA2G Buffer A (5X, with 1.5 mM MgCl2 at 1X) or KAPA2G Buffer M (5X, MgCl2-free), and MgCl2(25 mM).  Kits are available with and without dNTP Mix (10 mM each).

ReadyMix formulations (2X) of KAPA2G Fast and KAPA2G Fast HotStart DNA Polymerase are also available. These contain DNA polymerase, reaction buffer (with and without dye), and MgCl2 (at a final concentration of 1.5 mM).

Specifications
Spec
 Description
Starting Material
 Genomic DNA, cDNA, plasmid, lambda
Input Amount

< 100 ng for genomic DNA, cDNA

< 10 ng for less complex DNA
Contents
Components
KAPA2G Fast Standard or HotStart DNA Polymerase (5 U/µL)
5X KAPA2G Buffer A with MgCl2
5X KAPA2G Buffer M (Mg-free; available with select HotStart kits)
MgCl2 (25 mM)
dNTP Mix (10 mM each dNTP; optional)

For Research Use Only. Not for use in diagnostic procedures.
KAPA is a trademark of Roche.