MagNA Pure 96 Instrument

Your key to automated nucleic acid extraction

The MagNA Pure 96 System is a high-throughput instrument for automated nucleic acid purification. Typical run duration is less than one hour for 96 standard volume samples. Applications are very flexible, due to a diversity of software purification protocols using barcoded, prefilled trays with ready-to-use reagents.

MagNA Pure 96 kits are designed profiting from our more than ten year expertise in magnetic glass particle technology. We have streamlined the purification process to a very small number of reagents, consumables and steps.

Optimized preprogrammed purification protocols ensure routine sample handling and high quality, reproducible nucleic acid yields. Choose the appropriate purification protocol for your sample material and target nudeic acid:

 Target: Bacterial, fungal and viral NA
Protocol name Input sample type and sample volumes Elution   volume Run time**
(h:mm)
 Whole  blood Plasma   (citrate)   Plasma   (EDTA)    Serum  Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured  cells  Fresh-frozen  tissue   FFPET 
Pathogen Universal 2001 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL 200 μL       50,
100 μL
1:00
Viral NA Plasma SV1     50, 100,
200 μL
                    50,
100 μL
1:00
Viral NA Plasma ext lys SV1
Protocol optimized with external lysis reagent
100, 200 μL 100, 200 μL 100, 200 μL 100, 200 μL                   50,
100 μL
1:00
Viral NA Universal SV1 50, 100, 200 μL 50, 100, 200 μL   50, 100, 200 μL                   50,
100 μL
1:00
Pathogen Universal 5002
500 μL
500 μL
500 μL 500μL 500 μL 500 μL 500 μL 500 μL 500 μL 500 μL       50,
100 μL
1:30
Pathogen Universal 10002
1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL 1000 μL       50,
100 μL
1:40
Viral NA Plasma LV2       500 μL
                    50,
100 μL
1:10
Viral NA Plasma LV 10002       1000 μL
                    50,
100 μL
1:20

Viral NA Plasma ext lys LV2
Protocol optimized with external lysis reagent

  500 μL
500 μL
500 μL
                  50,
100 μL
1:10
Viral NA Universal LV2   500 μL
  500 μL
                  50,
100 μL
1:30
Viral NA Universal LV 10002     1000 μL
  1000 μL
                  50,
100 μL
1:30

1 MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001)
2 MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)

 *BAL - Bronchoalveolar Lavage
 **These times are approximate and should only be used as a guidance.

To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), and MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001). Please refer to the Instructions for Use manual for more details.


   Target: Genomic DNA
 Protocol name  Input sample type and sample volumes Elution   volume Run time**
(h:mm) 
 Whole blood Plasma (citrate)  Plasma (EDTA)  Serum  Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured cells Fresh-frozen tissue  FFPET 
DNA Blood ds SV1,a
Protocol optimized for double-stranded DNA and NGS
200 μL
                        50,
100 μL
1:00
DNA Blood SV1 50, 100, 200 μL
                        50,
100 μL
1:00
DNA Blood ext lys SV1
Protocol optimized with external lysis reagent
50, 100, 200 μL
50, 100, 200 μL
50, 100, 200 μL
50, 100, 200 μL
                  50,
100 μL
0:50
DNA Cells SV1                     Up to 5x105 cultured cells in 200 μL PBS     100 μL 1:00
DNA Tissue SV1                       Up to 5mg in 200 μL   100, 200 μL 1:20
DNA FFPE SV1                         1x5-10 um   50, 100 μL 1:00
DNA Blood LV2 500 μL                         100,
200 μL
1:20
DNA Blood LV 10002 1000 μL                         100,
200 μL
1:30
DNA Blood ext lys LV2
Protocol optimized with external lysis reagent
500 μL
500 μL
500 μL
500 μL
                  100,
200 μL
0:50

DNA Cells LV1

                    Up to 1x106 cultured cells in 200 μL PBS     100,
200 μL
1:20
DNA Tissue LV2                     Up to 25 mg
in 200 μL
    200 μL 1:20

 1 MagNA Pure 96 DNA and Viral NA Small Volume Kit (cat. no. 06 543 588 001)
 2
MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)
 4 New Protocols

 *BAL - Bronchoalveolar Lavage
 **These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details.


   Target: Cell-free NA
 Protocol name  Input sample type and sample volumes Elution   volume Run time**
(h:mm) 
 Whole blood Plasma (citrate)  Plasma (EDTA)  Serum  Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured cells Fresh-frozen tissue  FFPET 
cfNA ss 20002
Protocol optimized for single-stranded DNA
 
  2000 μL                     50,
100 μL
2:00
cfNA ss 40002
Protocol optimized for single-stranded DNA
 
  4000 μL                     50,
100 μL
2:20
cfNA ss 20002
Protocol optimized for double-stranded DNA
 
 
2000 μL
                    100,
200 μL
2:20
cfNA ss 40002
Protocol optimized for double-stranded DNA
    4000 μL                     100,
200 μL
2:50

 2 MagNA Pure 96 DNA and Viral NA Large Volume Kit (cat. No. 06 374 891 001)

 *BAL - Bronchoalveolar Lavage
 **These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details.


   Target: Total RNA
 Protocol name  Input sample type and sample volumes Elution   volume Run time**
(h:mm) 
 Whole blood Plasma (citrate)  Plasma (EDTA)  Serum  Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured cells Fresh-frozen tissue  FFPET 
Cellular RNA LV3  
                  Up to 1x106
cultured cells
in 200 μL PBS
    50,100,
200 μL
1:30
RNA Blood LV 4003 400 μL
                        50, 100,
200 μL
1:30
RNA Blood LV 8003 800 μL
                        100,
200 μL
1:30
RNA PAXgene LV3 PAXgene pallet in 400 μL PBS
                        100,
200 μL
1:30
RNA PAXgene Half Tube LV3 Half PAXgene pallet in 200 μL PBS
                        50,
100 μL
1:30
RNA Tissue FF Standard LV3  
                    Up to 10 mg
in 350 μL
  50,100,
200 μL
1:30
RNA Tissue FF High LV3  
                    Up to 25 mg
in 700 μL
  100, 200
μL
1:30
RNA Tissue FFPE LV3  
                      1x5-10μm
section
50,
100 μL
1:30

 3 MagNA Pure 96 Cellular RNA Large Volume Kit (cat. No. 05 467 535 001)

 *BAL - Bronchoalveolar Lavage
 **These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details.


   Target: Liquid handling
 Protocol name  Input sample type and sample volumes Elution   volume Run time**
(h:mm) 
 Whole blood Plasma (citrate)  Plasma (EDTA)  Serum  Swabs  BAL  Sputum   CSF   Urine   Stool   Cultured cells Fresh-frozen tissue  FFPET 
Sample Transfer to the
processing cartridge
 
                          N/A
Archiving Plate Transfer
For archiving purposes
 
        5 μL to 200 μL                 N/A
PCR Setup with Mixing  
        5 μL to 200 μL                 N/A
PCR Setup without Mixing  
        5 μL to 200 μL                 N/A
 *BAL=Bronchoalveolar Lavage
 **These times are approximate and should only be used as a guidance.
To get better results, Roche provides various External Lysis Buffers: MagNA Pure cfNA Buffer Set ( Cat. No. 07 794 398 001), MagNA Pure 96 External Lysis Buffer (Cat. No. 06 374 913 001), MagNA Pure 96 Bacterial Lysis Buffer (Cat. No. 06 374 921 001), MagNA Pure 96 DNA Tissue Lysis Buffer (Cat. No. 06 640 702 001), and MagNA Pure FFPET Buffer Set (Cat. No. 08 447 144 001). Please refer to the Instructions for Use manual for more details.