Specifications and Comparison

Product Name KAPA RNA HyperPrep Kits KAPA mRNA HyperPrep Kits KAPA RNA HyperPrep Kits with RiboErase (HMR) KAPA RNA HyperPrep Kits with RiboErase (HMR) Globin
Library Preparation Duration 4 hours 5.5 hours 6.5 hours 6.5 hours
Sample Input Amount 1-100 ng into library prep 50 ng - 1 µg into mRNA capture 25 ng - 1 µg into rRNA depletion 25 ng - 1 µg into rRNA depletion
Sample Types

High-quality total RNA

Degraded or FFPE total RNA

Poly(A)-selected and/or depleted RNA

High-quality total RNA

High-quality total RNA

Degraded or FFPE total RNA

High-quality total RNA

Degraded or FFPE total RNA

Whole blood total RNA

Species

Eukaryotic (Animal, Plant, etc.)

Prokaryotic (Bacteria, etc.)

Viral

Eukaryotic (Animal, Plant, etc.)

Human, Mouse, and Rat

Other species can be supported via KAPA RiboDesigner custom rRNA depletion

Human, Mouse, and Rat

Other species can be supported via KAPA RiboDesigner custom rRNA depletion

Stranded RNA-sequencing Yes Yes Yes Yes
Amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

KAPA HiFi DNA Polymerase

Compatible with low bias amplification

Reaction Purification KAPA Pure Beads included KAPA Pure Beads included KAPA Pure Beads included KAPA Pure Beads included
Multiplexing Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately Yes.  KAPA Adapters are not supplied, but available separately
Applications

Whole transcriptome sequencing

Gene expression with target enrichment

Splice junction and gene fusions with target enrichment

Gene expression

Annotation

Splice junction and gene fusions

Whole transcriptome

Long non-coding RNA Annotation

Gene expression

Splice junction and gene fusions

Whole transcriptome

Long non-coding RNA Annotation

Gene expression

Splice junction and gene fusions

Automation Friendly Yes.  Demonstrated and verified on many liquid handling systems.  Learn more Yes.  Demonstrated and verified on many liquid handling systems.  Learn more Yes.  Demonstrated and verified on many liquid handling systems.  Learn more Yes.  Demonstrated and verified on many liquid handling systems.  Learn more
Genialis™ Data Analysis and Visualization Solution Yes   Learn more Yes   Learn more Yes   Learn more Yes   Learn more
KAPA RiboDesigner Custom rRNA Depletion Support N/A N/A Yes   Learn more Yes   Learn more
Compatible Sequencing Platforms Illumina Sequencers Illumina Sequencers Illumina Sequencers Illumina Sequencers
Storage Information

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

Enzymes and buffers can be stored for up to 10 months at -20°C

 

KAPA Pure Beads can be stored for up to 10 months at 4°C

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