- Includes all reagents for efficient enzymatic depletion of globin mRNA from blood-derived RNA samples from high- or low-quality RNA
- Includes reagents for the depletion of human, mouse, or rat (HMR) rRNA
KAPA RNA HyperPrep Kits with RiboErase (HMR) Globin simultaneously removes rRNA and globin mRNA from blood-derived RNA. Together, effective RNAase H depletion and highly efficient library construction result in fewer non-informative reads (A) compared to the workflow from Supplier I, which employs bead-based depletion (orange). This translates to more complex libraries (B) and a larger number of unique transcripts detected (C).
Libraries were prepared from different inputs of RNA extracted from human blood, as indicated on the
x-axis of each graph. The 25 ng input is lower than the recommended minimum input for the Supplier I workflow. Paired-end (2 x 125 bp) sequencing was performed on an Illumina® HiSeq® 2500 instrument. Data were sub-sampled to 17 M reads per sample for analysis. Each bar represents the average of three technical replicates. Transcript abundance was quantified using Kallisto. To assess off-target depletion, transcript abundances were aggregated at the gene level and TMM-normalized prior to differential expression analysis. The expression profiles of libraries generated with or without globin depletion were compared to assess off-target depletion for each workflow.