1. Roche Sequencing | United States
  2. Library Preparation
  3. RNA Library Preparation
  4. KAPA RNA HyperPrep Kit with RiboErase (HMR)

KAPA RNA HyperPrep Kit with RiboErase (HMR)

Overview

The KAPA RNA HyperPrep Kit with RiboErase (HMR) targets and depletes rRNA using DNA probes and RNase H to provide improved coverage of transcripts of interest, including precursor mRNAs and important regulatory species, such as noncoding RNAs. Sequencing of rRNA-depleted total RNA samples provides a more comprehensive representation of the whole transcriptome. This kit is suitable for total RNA input from human, mouse and rat species only.

Benefits of KAPA RNA HyperPrep Kits with RiboErase

• rRNA depletion and purification in ~2.5 hours

• Robust performance across different sample types and input amounts

• High success rates with low-input and degraded samples

• Qualified automation methods

 

Product Highlights

Sequence what matters

  • Waste fewer reads on rRNA carryover and PCR duplicates
  • Identify more unique transcripts and genes with equivalent sequencing

View comparative data showing a reduction in wasted reads due to alignment to rRNA loci and PCR duplicates, and data showing increased number of genes and transcripts, using the KAPA RNA HyperPrep Kit with RiboErase compared to Illumina TruSeq Stranded Ribo-Zero Gold or NEBNext Ultra Directional with rRNA Depletion Kits.

 

Achieve superior coverage uniformity

  • Obtain more uniform distribution of reads across transcripts
  • Improve coverage of difficult GC-rich transcripts

View data on even coverage across transcript lengths, and comparative data showing improved coverage of GC-rich regions of SLC2A4RG, using the KAPA RNA HyperPrep Kit with RiboErase compared to Illumina TruSeq Stranded Ribo-Zero Gold or NEBNext Ultra Directional with rRNA Depletion Kits.

 

Generate high-quality libraries from degraded samples

  • Prepare libraries with as little as 25 ng  FFPE RNA samples, depending on total RNA quality
  • Achieve low duplication rates and highly efficient, reproducible rRNA removal with degraded samples
  • Identify more unique transcripts and genes with equivalent sequencing

View data showing fewer reads wasted due to PCR duplicates and alignment to rRNA loci compared to competitor kits, and data showing higher number of genes and unique transcripts identified from FFPE samples, using the KAPA RNA HyperPrep Kit with RiobErase compared to Illumina TruSeq Stranded Ribo-Zero Gold or NEBNext Ultra Directional with rRNA Depletion Kits. 


Technical Documents

Instructions for product use and other product-related technical documents

 

Research Use Only. Not for use in diagnostic procedures.
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