Specifications

Kits can be stored for up to 12 months at -20˚C.

Complete kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (10X) and a set of 6 DNA Standards. Reagents for qPCR (KAPA SYBR FAST qPCR Master Mix and Primer Premix) and DNA Standards (1–6) are also sold separately. Where noted, Master Mixes contain instrument-specific reference dyes, while the Universal kit includes ROX High and ROX Low (both 50X) separately.

Specifications

Spec	Description
Compatible Platforms	Illumina HiSeq, NextSeq, MiSeq, and GAIIx Ion Torrent Proton and PGM 454 GS FLX+ and GS Junior SOLiD 5500 series
Library Type	Any
Starting Material	Any NGS library ready for cluster amplification or emPCR
Standard curve concentration range	20–0.0002 pM for Illumina libraries 83–0.00083 pM for Ion Torrent and 454 libraries 10–0.0001 pg/µL for SOLiD libraries
Sequencing Applications	Whole Genome Sequencing, Whole Exome Sequencing, Targeted Sequencing (custom panels), RNA-Seq, ChIP-Seq, Amplicon Sequencing, Methyl-Seq

Components–Illumina Platform

	Kit code	10X Illumina Primer Premix	2X KAPA SYBR FAST qPCR Master Mix (universal)	50X KAPA SYBR FAST ROX High	50X KAPA SYBR FAST ROX Low	2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye)	2X KAPA SYBR FAST qPCR Master Mix (ROX Low)	Illumina DNA Standards 1-6
Complete Kit (universal)	KK4824	X	X	X	X			X
Complete Kit (with equipment-specific dye)	KK4835 KK4844 KK4854	X				X		X
Complete Kit (ROX Low)	KK4873	X					X	X
qPCR MasterMix (universal) and Primer Premix only	KK4923	X	X	X	X
qPCR MasterMix (with equipment-specific reference dye) and Primer Premix only	KK4933 KK4943 KK4953	X				X
qPCR MasterMix (ROX Low) and Primer Premix only	KK4973	X					X

Components–Ion Torrent Platform

	Kit Code	10X Illumina Primer Premix	2X KAPA SYBR FAST qPCR Master Mix (universal)	50X KAPA SYBR FAST ROX High	50X KAPA SYBR FAST ROX Low	2X KAPA SYBR FAST qPCR Master Mix (with equipment-specific dye)	2X KAPA SYBR FAST qPCR Master Mix (ROX Low)	Illumina DNA Standards 1-6
Complete Kit (universal)	KK4827	X	X	X	X			X
Complete Kitq (with equipment-specific dye)	KK4838 KK4847 KK4857	X				X		X
Complete Kit (ROX Low)	KK4874	X					X	X
qPCR MasterMix (universal) and Primer Premix only	KK4924	X	X	X	X
qPCR MasterMix (with equipment-specific reference dye) and Primer Premix only	KK4934 KK4944 KK4954	X				X
qPCR MasterMix (ROX Low) and Primer Premix only	KK4974	X					X

Research Use Only. Not for use in diagnostic procedures.
KAPA and SEQCAP are trademarks of Roche. Other product names and trademarks are the property of their respective owners.

KAPA Library Quantification Kits