KAPA Adapters for
Illumina Sequencing

KAPA Unique Dual-Index (UDI) and KAPA Dual-Indexed (DI) Adapter Kits contain full-length, ready-to-use, QC-tested, dual-indexed Illumina® adapters for ligation-based library construction. Kit specifications are compared and contrasted below.


For additional details related to product shipping, storage and handling, please refer to technical documentation.

  KAPA Unique Dual-Indexed Adapters KAPA Dual-Indexed Adapters
Recommended for instruments with non-patterned flow cells
(e.g. MiSeq 3000, NextSeq 4000, HiSeq 2000, HiSeq 2500)
Yes Yes
Recommended for instruments with patterned flow cells
(e.g. HiSeq 3000, HiSeq 4000, HiSeq X, Novaseq)
Yes No
Designed to mitigate index assignment
Yes No
Number of full-length, dual-indexed adapters
96 96
Nature of dual sequencing indexes
Unique Combinational
Number of 8-nt non-reduntant p5 barcodes 96 8
Number of 8-nt non-reduntant p7 barcodes 96 12
Barcodes identical to those used in adapters supplied by illumnia No (a) Yes
Compatible with KAPA HyperPrep, KAPA HyperPlus, KAPA RNA
HyperPrep and HyperCap workflows
Yes Yes
Compatible with Roche Universal Blocking Ollgos
Yes Yes
Compatible with methyl-seq applications (b)
No No
Adapter formulation Ready-to-use, 15 mM solutions; 20mL per adapter (c)
Kit configuration Hard-shell plate with automation-friendly labelling
and overfills and replacements seats (d)
KAPA Adapter Dilution Buffer included Yes (25 mL per kit)   
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(a) The set of 192 barcode sequences used in KAPA UDI Adapters is exclusive to Roche.
(b) SeqCap adapters are methylated and may be used in combination of KAPA library construction kits in bisulfite sequencing workflows that are that require the ligation of methylated adapters during library construction.
(c) This is sufficient for four library preps with the KAPA HyperPrep or KAPA HyperPlus Kit if no adapter dilution is required. Generous overfill supports use on automated liquid handling system.
(d) KAPA Adapter plates are shipped with peelable seals. Replacement seals for KAPA UDI Adapter Kits are peelable and pierceable whereas replacement seals for KAPA DI Adapters Kits are peelable only.