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  2. Library Preparation
  3. Library Amplification
  4. KAPA HiFi Kits
  5. KAPA Library Amplification Kits

KAPA Library Amplification Kits

Kits can be stored for up to 12 months at -20˚C.

Kits include KAPA HiFi HotStart ReadyMix (2X), a convenient PCR master mix containing KAPA HiFi HotStart DNA Polymerase, KAPA dNTPs, reaction buffer, and MgCl2 at a final concentration of 2.5 mM. Kits with primers are also available.

Kits for real-time library amplification include a real-time version of the KAPA HiFi HotStart ReadyMix, as well as fluorescent standards to monitor library amplification.

Specifications
Spec     Description
Compatible Platform Illumina HiSeq, MiSeq, NextSeq, and GAIIx
Library Type
 DNA, RNA, and bisulfite-treated DNA
Starting Material Any NGS library that requires amplification
Sequencing Applications
 Whole Genome Sequencing, Whole Exome Sequencing,  Targeted Sequencing (custom panels), RNA-Seq, ChIP-Seq, Amplicon Sequencing
Components
  Kit Code   2XKAPA HiFi HotStart ReadyMix 2X KAPA HiFI HotStart Real time PCR Master Mix Fluorescein Primers
Standard Kit

KK2611

KK2612

                   X      
With primer mix

KK2620

KK2621

                    X           X
Real time PCR lbrary amplification with fluoroscent standards

KK2701

KK2702

  

                                   

                                  X

         

       X

  


Technical Documents

Instructions for product use and other product-related technical documents

Research Use Only. Not for use in diagnostic procedures.
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