1. Roche Sequencing
  2. Library Preparation
  3. Library Amplification
  4. KAPA Library Amplification Kits
  5. Specifications

KAPA Library Amplification Kits

Store kits for 18 months at -20°C.

Kits include KAPA HiFi or KAPA HiFi HotStart DNA Polymerase (1 U/µL), Fidelity Buffer (5X), GC Buffer (5X), high-quality dNTPs (10 mM each) and MgCl2 (25 mM). Both buffers contain MgCl2 at a final concentration of 2 mM.

KAPA HiFi HotStart is also available in KAPA HiFi HotStart ReadyMix (2X), which contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM).

Specifications
Spec Description  
Compatible Platform Illumina HiSeq, MiSeq, NextSeq, and GAIIx
Library Prep DNA, RNA, and bisulfite-treated DNA
Starting Material Any NGS library that requires amplification
Sequencing Applications Whole Genome Sequencing, Whole Exome Sequencing,  Targeted Sequencing (custom panels), RNA-Seq, ChIP-Seq, Amplicon Sequencing
Components
Spec Kit Code 2XKAPA HiFi HotStart ReadyMix 2X KAPA HiFI HotStart Real time PCR Master Mix Fluorescein Primers
Standard Kit

KK2611

KK2612

 X      
With primer mix

KK2620

KK2621

 X     X
Starting Material

KK2701

KK2702

   X X  

Research Use Only. Not for use in diagnostic procedures.
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