1. Roche Sequencing
  2. Library Preparation
  3. Library Amplification
  4. KAPA HiFi Kits
  5. Resources for CRISPR/Cas9 genome editing
Image of a pair of tweezers removing a piece of DNA strand, placed on the right with the text - CRISPR Gene Editing

Resources for CRISPR/Cas9-based genome editing

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The Application of Next-genaration Sequencing in CRISPR Gene Editing and Single-cell Analysis

Accurate DNA amplication is essential for NGS-based workows, including CRISPR gene editing, screening of CRISPR clones for on-target and off-target genome modications, and single-cell RNA sequencing (scRNA). 

In this webinar, we demonstrate how KAPA HiFi DNA polymerase and KAPA NGS Library Preparation Kits enable robust workows for:

  • CRISPR/Cas-9 technology for genome editing
  • Indel screening following CRISPR/Cas-9 editing
  • CIRCLE-seq, a QC methodology for identifying CRISPR/Cas-9 off-target cleavage sites
  • Single-cell RNA sequencing (scRNA-seq)

Download CRISPR amplicon screening application note

CRISPR workflow

Targeted CRISPR indel screening using an automated KAPA HyperPrep workflow

This app note discusses:

  • Development of an automated, high-throughput CRISPR screening workflow using the KAPA HyperPrep Kit
  • Automated library preparation for CRISPR sequencing with minimal QC or sample-specific workflow modifications, enabling a rapid turnaround time
  • Compatibility of the KAPA HyperPrep workflow with a wide range of amplicon input amounts and sizes

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Learn more about Roche reagents for your CRISPR workflow

KAPA HiFi Kits

Reliable and accurate DNA amplification is critical to the success of many molecular biology applications, especially senstive methods where small errors can dramatically alter the outcome. Generation of CRISPR/Cas9 targeting materials with KAPA HiFi reduces sequence errors, increasing the accuracy of both cleavage and insertion of the intended sequence.

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KAPA HyperPrep Kits

The KAPA HyperPrep Kit provides a streamlined, versatile library preparation solution that significantly reduces sequencing library preparation time. The novel, single-tube chemistry offers improvements to library construction efficiency, particularly for challenging samples such as FFPE and cell-free DNA.

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Application Highlights

CIRCLE-seq for CRISPR/Cas9 Off-target Screening

KAPA HiFi CRISPR gene editing and validation screening

Research Use Only. Not for use in diagnostic procedures.
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