The Application of Next-genaration Sequencing in CRISPR Gene Editing and Single-cell Analysis
Accurate DNA amplication is essential for NGS-based workows, including CRISPR gene editing, screening of CRISPR clones for on-target and off-target genome modications, and single-cell RNA sequencing (scRNA).
In this webinar, we demonstrate how KAPA HiFi DNA polymerase and KAPA NGS Library Preparation Kits enable robust workows for:
- CRISPR/Cas-9 technology for genome editing
- Indel screening following CRISPR/Cas-9 editing
- CIRCLE-seq, a QC methodology for identifying CRISPR/Cas-9 off-target cleavage sites
- Single-cell RNA sequencing (scRNA-seq)