LightCycler­® 96 Instrument

High-performance real-time PCR

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General

Weight

approx. 55 lb (25 kg)

Dimensions

W x D x H: 16 in x 16 in x 21 in (40 cm x 40 cm x 53 cm)

Noise level during run

43 dB(A)

Hardware

Thermal cycling system

Peltier-based, 96-well block

Max ramp rate heating

4.4°C/s

Average ramp rate cooling

2.2°C/s

Programmable temperature range

37-98°C

Temperature accuracy

±0.2°C of programmed target temperature

Tm uniformity

Range (max-min) 0.4°C; STD <0.1

Gradient function

Yes

Gradient operational range

37-98°C

Gradient programmable span (X-Y °C)

Max. 20°C gradient range

Excitation

High-power broad spectrum LED

Measurement (Integration time)

Simultaneous data acquisition for all positions in 10-1000 ms (dynamic mode)

Detection

CCD Camera

Optical system

Fixed fiber optics with four excitation and four emission filters

No scanning technologies

Cq uniformity

Range (max-min) 0.8; STD <0.2 (enabling resolution of 2-fold concentration differences)

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Run Mode

Standalone

Touchscreen support for instrument configuration

Flexible experiment programming and execution

Online fluorescence display

PC connected

PC monitoring, analysis tools, and programming

LAN connected

Support of online monitoring using LAN connection

Support of remote Roche Service

External devices

Support of external barcode scanner using USB connection

Instrument active communication

Email notification, with success or failure messaging and optional experiment file attachment

Electrical approvals

CE, ICE, UL

Reaction volumes

10-50 μl

Sample format

96-well, 8-tube strips

Runtime

<40 min for 3-step, 40-cycle PCR

Melting measurement

Continuous with 1-25 data points/°C

Applications

Licensed for real-time PCR

Yes

Dynamic range

10 orders of magnitude

Range of excitation/emission wavelengths (X-Y nm)

470/514 (SYBR, FAM, ResoLight dye)

533/572 (VIC, Hex, Yellow555)

577/620 (Red610, Texas Red)

645/697 (Cy5)

Detection formats

Intercalating dyes (SYBR Green I, ResoLight dye): Hydrolysis Probes

Multiplex analysis (e.g., up to X targets per well)

Up to 4 channels, pre-calibrated color compensation (no user interaction necessary) All channels can be viewed by the software in one chart simultaneously or viewed in 4 charts separately on one screen

Additional dye available

For mono color experiments, any dye between 500 and 720 nm emission wavelength can be used

For dual color experiments, only pre-calibrated dyes are usable

Passive reference dyes (e.g., Rox) necessary

No

Analysis Software

Operating systems

Win 7 or Win XP

Data analysis

Absolute Quantification

Relative Quantification

Tm Calling

Endpoint Genotyping

Qual Detection *

HRM *

(* Coming Soon)

Data export

Result table: export as .txt file Charts: Data and image export

Data import

Sample information can be imported from Excel, .csv, or .txt files

Failure flagging

Automated flagging of critical controls (e.g., positive control is negative or negative control is positive)

User definable flagging for further criteria (e.g., standard curve with error greater than X)