General |
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Weight |
approx. 55 lb (25 kg) |
Dimensions |
W x D x H: 16 in x 16 in x 21 in (40 cm x 40 cm x 53 cm) |
Noise level during run |
43 dB(A) |
Hardware |
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Thermal cycling system |
Peltier-based, 96-well block |
Max ramp rate heating |
4.4°C/s |
Average ramp rate cooling |
2.2°C/s |
Programmable temperature range |
37-98°C |
Temperature accuracy |
±0.2°C of programmed target temperature |
Tm uniformity |
Range (max-min) 0.4°C; STD <0.1 |
Gradient function |
Yes |
Gradient operational range |
37-98°C |
Gradient programmable span (X-Y °C) |
Max. 20°C gradient range |
Excitation |
High-power broad spectrum LED |
Measurement (Integration time) |
Simultaneous data acquisition for all positions in 10-1000 ms (dynamic mode) |
Detection |
CCD Camera |
Optical system |
Fixed fiber optics with four excitation and four emission filters No scanning technologies |
Cq uniformity |
Range (max-min) 0.8; STD <0.2 (enabling resolution of 2-fold concentration differences) |
Run Mode |
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Standalone |
Touchscreen support for instrument configuration Flexible experiment programming and execution Online fluorescence display |
PC connected |
PC monitoring, analysis tools, and programming |
LAN connected |
Support of online monitoring using LAN connection Support of remote Roche Service |
External devices |
Support of external barcode scanner using USB connection |
Instrument active communication |
Email notification, with success or failure messaging and optional experiment file attachment |
Electrical approvals |
CE, ICE, UL |
Reaction volumes |
10-50 μl |
Sample format |
96-well, 8-tube strips |
Runtime |
<40 min for 3-step, 40-cycle PCR |
Melting measurement |
Continuous with 1-25 data points/°C |
Applications |
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Licensed for real-time PCR |
Yes |
Dynamic range |
10 orders of magnitude |
Range of excitation/emission wavelengths (X-Y nm) |
470/514 (SYBR, FAM, ResoLight dye) 533/572 (VIC, Hex, Yellow555) 577/620 (Red610, Texas Red) 645/697 (Cy5) |
Detection formats |
Intercalating dyes (SYBR Green I, ResoLight dye): Hydrolysis Probes |
Multiplex analysis (e.g., up to X targets per well) |
Up to 4 channels, pre-calibrated color compensation (no user interaction necessary) All channels can be viewed by the software in one chart simultaneously or viewed in 4 charts separately on one screen |
Additional dye available |
For mono color experiments, any dye between 500 and 720 nm emission wavelength can be used For dual color experiments, only pre-calibrated dyes are usable |
Passive reference dyes (e.g., Rox) necessary |
No |
Analysis Software |
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Operating systems |
Win 7 or Win XP |
Data analysis |
Absolute Quantification Relative Quantification Tm Calling Endpoint Genotyping Qual Detection * HRM * (* Coming Soon) |
Data export |
Result table: export as .txt file Charts: Data and image export |
Data import |
Sample information can be imported from Excel, .csv, or .txt files |
Failure flagging |
Automated flagging of critical controls (e.g., positive control is negative or negative control is positive) User definable flagging for further criteria (e.g., standard curve with error greater than X) |