LightCycler­® 96 Instrument

High-performance real-time PCR

Request a Quote

Innovative. Intuitive. Accurate. Dependable.

For today's academic research labs, the new Roche LightCycler® 96 System is the optimal mid-size, mid-throughput real-time PCR solution that conveniently delivers the highest standards expected from a LightCycler® System: An ideal combination of accuracy, temperature homogeneity, and reproducibility, matched with powerful software so intuitive that it is accessible to any user.

The LightCycler® 96 Instrument is a real-time PCR instrument for rapid cycling up to 96 samples. Applications include absolute and relative quantification, melting curve analysis, and endpoint genotyping.

Precise LightCycler® 96 Instrument thermal homogeneity and cycling speed produce accurate and reproducible results in a very short time.

The LightCycler® 96 Instrument’s optical detection system flexibly detects sequence-dependent probes (i.e., hydrolysis probes) and sequence-independent dyes (i.e., SYBR Green I). Multiplex and multicolor capabilities permit the use of up to four different fluorescent dyes*.

The innovative LightCycler® 96 Application and Instrument Software creates a simple analysis workflow with easy import and export functions, email notifications after each run, online monitoring flexibility, and server-based network interfacing.


  • Plate-based real-time PCR instrument with 96-well block
  • Temperature gradient (assay optimization)
  • Standalone system (integrated PC)
  • Touchscreen with separate instrument software 
  • Dynamic range of 10 log
  • Sensitivity down to one copy
  • 4-plex multicolor
  • Runtime of less than 1 hour
  • Applications: Absolute Quantification, Relative Quantification, Endpoint Genotyping, Melting Curve Analysis, High Resolution Melting
  • Intuitive state-of-the-art license-free software

Obtain fast, highly sensitive, and reproducible results for qualitative and quantitative detection of nucleic acids and SNP analysis with the versatile, plate-based LightCycler® 96 Instrument:

  • Benefit from a cutting-edge, low-mass thermal block design that offers excellent well-to-well homogeneity for a broad range of temperatures, as well as access to gradient features. The electroforming production process allows a perfect fit of the 96-well plate (negative structure) to the block (positive structure, formed by a galvanic reaction) and avoids air cushions in between both.
  • Maximize sensitivity and speed using novel optics (sturdy glass fibers combined with high-intensity broad-spectrum LEDs), and obtain simultaneous detection of all wells within milliseconds.
  • Install the software on a separate computer with no license requirements, and run the system according to your specific needs:
    • Standalone, using a flash drive to transfer data
    • Connected to a PC
    • Connected to a PC network via Ethernet
  • Experience advanced software features, such as on-line real-time remote monitoring, e-mail notification, heat-maps, wizards, and many more options to operate the intuitive and easy-to-use software.
  • Extract meaningful data via state-of-the-art analysis methods, then easily export results in a ready-to-publish format (RDML).
  • Choose the optimal type of LightCycler® 480 Multiwell Plate or 8-Tube strips to best fit your assay format, application, and workflow. Both white (recommended) and clear formats are available.