LightCycler­® 480 Instrument II

Rapid high-throughput, plate-based real-time PCR amplification and detection instrument.

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LightCycler­® 480 Instrument II

Dimensions

W x D x H: 24 in x 24 in x 21.5 in (57.4 cm x 58.8 cm x 49.7 cm)

Weight

121 lb (55.6 kg)

Power consumption

200–240 Vac (50/60 Hz, 1500 VA)

Reaction volumes

5 μL–20 μL (384-well)

10 μL–100 μL (96-well)

Temperature control

Peltier-based heating/cooling from 37°C–95°C (20° starting temperature to perform specific melting curve analyses) 

Heating rate

96-well block: 4.4°C/s

384-well block: 4.8°C/s

Cooling rate

96-well block: 2.2°C/s

384-well block: 2.5°C/s

Excitation

LightCycler® 480 LED Lamp (430-630 nm)

Detector

Cooled monochrome CCD camera

Filters

Excitation (nm): 440, 465, 498, 533, 618

Detection (nm): 488, 510, 580, 610, 640, 660

Computer

Pentium PC with Windows 7

Preinstalled software configuration

  • Absolute Quantification
  • Relative Quantification Analysis
  • Tm Calling
  • Endpoint Genotyping
  • Absolute Quantification Analysis
  • Melting Curve Genotyping

Accessory software 

analysis module

Gene Scanning Software

Automation

  • Interface with LightCycler® 480 LIMS Interface Module
  • Barcode-assisted multiwell plate scanning
  • Plate loading capability

Data management

21 CFR Part 11 compatibility

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