Application
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Product
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Features and Benefits
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|---|
Cloning
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KAPA HiFi PCR Kits
KAPA Long Range PCR Kits
|
- High-fidelity amplification of inserts up to 10 kb with an error rate 100X lower than Taq DNA polymerase. Compatible with blunt-end cloning
- Amplification of inserts up to 15 kb with high sensitivity and an error rate 4X lower than Taq DNA polymerase. Compatible with TA cloning
|
Colony PCR
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KAPA2G Robust PCR Kits
KAPA HiFi HotStart PCR Kits
|
- Improved tolerance to PCR inhibitors results in higher success rates and streamlined workflows with common E. coli and S. cerevisae strains*
- Recommended for colony PCR when high fidelity and/or longer targets up to 10 kb are required
|
Crude Sample PCR
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KAPA2G Robust PCR Kits
KAPA3G Plant PCR Kits
KAPA Mouse Genotyping Kits
|
- Improved resistance to many inhibitors commonly found in crude samples—such as denatured protein, salts and metabolites—results in higher PCR success rates and more streamlined workflows*
- Improved resistance to inhibitors co-purified in plant DNA extracts, and amplification directly from plant tissues enable higher-throughput workflows and eliminate the need for expensive and time-consuming purification*
- Increased throughput and decreased turnaround time*
- Better performance compared to other available crude extraction methods *
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Direct Plant PCR
|
KAPA3G Plant PCR Kits |
- Improved resistance to inhibitors co-purified in plant DNA extracts, and amplification directly from plant tissues enable higher-throughput workflows and eliminate the need for expensive and time-consuming purification*
|
Fast PCR
|
KAPA2G Fast PCR Kits |
- Higher specific activity and processivity significantly reduces PCR cycling times by 50% without a loss in performance*
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GC-rich PCR
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KAPA2G Robust PCR Kits
KAPA HiFi PCR Kits
|
- Recommended for the amplification of GC-rich targets
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Genotyping
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KAPA2G Robust PCR Kits
KAPA2G Fast PCR Kits
KAPA Taq PCR Kits
KAPA Mouse Genotyping Kits
|
- Robust performance, shorter cycling times, streamlined workflows and higher success rates across a wide variety of template types and amplicons*
- Reduced PCR cycling times and improved success rates streamline workflows for routine genotyping*
- High quality and optimal formulation for routine genotyping applications
- Increased throughput and decreased turnaround time*
- Better performance compared to other available crude extraction methods*
|
High-fidelity PCR
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KAPA HiFi PCR Kits
KAPA LongRange PCR Kits
|
- Error rate 100X lower than Taq DNA polymerase. Higher processivity results in significant improvements to fidelity, sensitivity, target length, and the ability to amplify difficult templates*
- Recommended for the amplification of longer targets up 15 kb with improved sensitivity and an error rate 4X lower than Taq DNA polymerase
|
Long-range PCR
|
KAPA LongRange PCR Kits
KAPA HiFi PCR Kits
|
- Recommended for the amplification of longer targets up 15 kb with improved sensitivity and an error rate 4X lower than Taq DNA polymerase*
- High-fidelity amplification of inserts up to 10 kb with an error rate 100X lower than Taq DNA polymerase*
|
Multiplex PCR
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KAPA2G Fast Multiplex Kits
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- Increased specific activity and processivity, formulated for highly sensitive and uniform multiplex amplification*
|
Single-protocol PCR
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KAPA2G Robust PCR Kits
KAPA2G Fast PCR Kits
|
- Robust performance across a wide range of GC- and AT-rich templates streamlines workflows while reducing turnaround times. Recommended for end-point applications using gel electrophoresis
- Broad coverage of both AT- and GC-rich targets consolidates protocols and reduces cycling times. Recommended for higher sensitivity detection such as capillary electrophoresis and Sanger sequencing workflows
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Site-directed Mutagenesis
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KAPA HiFi PCR Kits
|
- Error rate 100X lower than Taq DNA polymerase.* Higher processivity results in significant improvements to fidelity and target length.* Compatible with site-directed mutagenesis protocols
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