Short Talk: Analyzing the SARS-CoV-2 genome with target enriched RNA-seq
Date: Thursday, March 11, 2021
Time: 3:00 PM – 4:00 (EST)
Duration: 10-minute presentation
Session: New Technologies in Genomics & Imaging
Abstract:
RNA-seq with hybridization-based target enrichment (TE) enables high-throughput genome studies of RNA viruses such as SARS-CoV-2, which are critical for discovering new viral mutations and tracking strain transmission. We have developed a KAPA SARS-CoV-2 Target Enrichment Panel and workflow for SARS-CoV-2 genome sequencing. In this presentation, we discuss the performance of this panel on samples with varying levels of SARS-CoV-2 RNA in human total RNA. We also present a single-day target enrichment workflow with hybridization times as short as one hour. Finally, we will show that the KAPA SARS-CoV-2 Target Enrichment Panel can detect mutations from different strains of SARS-CoV-2 within a single reaction.
Sarah Trusiak, PhD
Senior Applications Scientist, Roche Sequencing and Life Science
Dr. Trusiak holds a Ph.D. in genetics and genomics from the University of Connecticut. She has spent the past ten years working in NGS, applying and developing NGS technology in academic and industry settings.