Check out our virtual poster in the CGC poster hall:
HyperPETE*: A Novel Primer Extension Target Enrichment Solution for Rapid and High-Performing NGS Results
Florence Crary-Dooley¹*, Liu Xi¹*, Nitya Furtado¹, Jingchuan Li¹, Latha Ramaswamy¹, Liesl Joubert², Bronwen Miller², David Penkler², Jo-Anne Penkler², Etienne Slabbert², Marius Snyman², Mingyang Cai¹, Liang Feng¹, Ruben van der Merwe¹, Brian Godwin¹
¹ Roche Sequencing Solutions, Pleasanton, CA 94588, ² Roche Sequencing Solutions, Cape Town, South Africa 7925, *contributed equally
We developed HyperPETE*, a novel target enrichment technology and reagent kit that employs primer extension reactions to specifically capture and release target library molecules for sequencing. The combined library preparation and enrichment steps can be performed within a single workday, result in high-performance coverage uniformity, detect all major somatic variant types (SNV, CNV, InDel, MSI status, and novel fusion transcript partners), and can be performed on tissue DNA, plasma DNA, and tissue RNA samples.
Here we show results from plasma DNA, FFPET DNA, and FFPET RNA samples processed with our HyperPETE* workflow and reagent kit† , and then analyzed with our in-house analysis pipeline. The HyperPETE* product† delivers the high performance of a hybridization capture technology with a faster turnaround time, enabling researchers to go from sample to answer rapidly and reliably.