SeqCap lncRNA Enrichment Kit

Roche_SeqCap_RNA_Launch_WebBanner_IncRNA_Update

Overview

The SeqCap lncRNA Enrichment Kits, as part of the SeqCap RNA Enrichment System, enables the targeting of lncRNAs (Long non-coding RNAs). These non-protein coding transcripts, longer than 200 nucleotides, are quite abundant. LncRNA has been reported associated with various diseases or biological processes.

It is estimated that there are 4 times as many lncRNAs as mRNA in the human transcriptome. The SeqCap lncRNA Enrichment Kits are designed to selectively capture lncRNAs to enable targeted study of this common, but not well understood, component of the human transcriptome.

Description Catalog Number Pack Size
SeqCap lncRNA Enrichment Kit 07277270001 4 Reactions
SeqCap lncRNA Enrichment Kit 07277288001 48 Reactions
SeqCap lncRNA Enrichment Kit 07277296001 384 Reactions

For life science research only. Not for use in diagnostic procedures.

Please Contact Us for ordering and questions


Description

The SeqCap RNA lncRNA Enrichment Kits are a fixed content catalog design that is designed to capture 17 Mb of long non-coding RNA (lncRNA) targets from hg19 annotation, representing 32,808 lncRNA and TUCP (transcripts with unknown coding potential) lncRNA transcript isoforms. Sources for the design are the Human Body Map Catalog and Gencode v19.

These kits come in 4, 48 and 384 reaction sizes. Each reaction can be used for multiple samples through pre-capture multiplexing. Talk to your local Roche representative to find out what multiplexing scope is right for you.

Benefits

  • Selectively target lncRNAs, a common, but not well understood, component of the human transcriptome
  • Achieve unprecedented depth and sensitivity for rare transcript detection with effectively conserved transcript abundance
  • Maximize the discovery and characterization of transcript variants and isoforms
  • Investigate the developmental regulation of lncRNAs
  • Investigate the tissue- and cell-type specificity of lncRNAs
  • Investigate the role of lncRNA in disease

Additional Information

Performance Data

figure1_kidney_liver_rna_600w

Figure 1: Greater isoform discovery with SeqCap RNA. Ambion Human Kidney Total RNA (left) and Human Liver Total RNA (right) were sequenced with standard RNA-Seq (black) and SeqCap RNA (red) to determine the number of lncRNA transcript isoforms detected when subsampled to various number of reads (x-axis). Captures were performed with the SeqCap lncRNA Enrichment Kit. SeqCap RNA data subsampled to 1 M reads detected more transcript isoforms than RNA-Seq data subsampled to 20 M reads, indicating >20 times increased discovery power or sequencing throughput.

Design Files

The design files provide information about genomic regions covered by the SeqCap lncRNA Enrichment Kit probes.

Download all design files

Overview

The SeqCap RNA lncRNA Enrichment Kits are a fixed content catalog design that is designed to capture 17 Mb of long non-coding RNA (lncRNA) targets from hg19 annotation, representing 32,808 lncRNA and TUCP (transcripts with unknown coding potential) lncRNA transcript isoforms.

File Descriptions

The folder contains these three files:

  • 140529_HG19_lnc_RNA_primary_targets.bed: This file contains primary target intervals along with associated annotation IDs in BED format.
  • 140529_HG19_lnc_RNA_capture_targets.bed: This file contains capture target intervals along with associated annotation IDs in BED format. The coordinates listed here correspond to locations where capture probes were actually designed and placed.
  • 140529_HG19_lnc_RNA_coverage_summary.txt: This file contains information regarding the statistics of probe coverage and capture regions.
  • ERCC_controls.bed: This file contains the ERCC controls in BED format.
  • ERCC_controls.fa: This FASTA file contains the sequences of the ERCC controls,
  • The BED files can be opened using SignalMap

Related Products

Description Pack Size Catalog Number
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KAPA Stranded RNA-Seq Library Preparation Kit 96 reactions 07277253001
SeqCap Adapter Kit A 96 reactions 07141530001
SeqCap Adapter Kit B
96 reactions
07141548001
SeqCap Hybridization and Wash Kit
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SeqCap Hybridization and Wash Kit 96 reactions 05634253001
SeqCap EZ Accessory Kit V2 24 reactions 07145594001
SeqCap EZ Accessory Kit V2 96 reactions 06776345001
SeqCap HE-Oligo Kit A
8 reactions x 12 oligo 06777287001
SeqCap HE-Oligo Kit B 8 reactions x 12 oligo 06777317001
SeqCap Pure Capture Bead Kit 24 reactions 06977952001