SeqCap EZ Exome + UTR

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Product Overview

The SeqCap EZ Human Exome + UTR Kit, as part of the SeqCap EZ Enrichment System, enables enrichment of 64 Mb of coding exons and miRNA regions, plus 32 Mb of untranslated regions (UTRs).

Description Catalog Number Pack Size
SeqCap EZ Exome+UTR 06740294001 4 Reactions
SeqCap EZ Exome+UTR 06740308001 48 Reactions

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New! SeqCap EZ HyperCap Workflow User's Guide v1.0 

The SeqCap EZ system can now be used with the SeqCap EZ HyperCap Workflow. The HyperCap workflow provides an integrated, application-specific approach for efficient, streamlined and automatable targeted sequencing solution. It combines high-efficiency KAPA Hyper Prep and KAPA HyperPlus Library Preparation Kits with performance-optimized SeqCap EZ capture protocol.

For life science research only. Not for use in diagnostic procedures.


Product Description

The SeqCap EZ Exome + UTR Kit is based on the same coding exon sources from SeqCap EZ Human Exome Kit v3.0 with expanded coverage of 5’- and 3’-untranslated regions (UTRs) from the following sources:

  • NCBI Reference Sequence (RefSeq) refGene table from UCSC GRCh37/hg19 March 2012
  • Ensembl (GRCh37 v64)

Benefits

  • The most comprehensive exome and UTR enrichment solution.
  • Minimize sequencing costs with the superior capture efficiency of our 2.1M overlapping probes.
  • Discover more variants compared to all other target enrichment solutions.

Design Files

Design & Annotation files were designed for use with the following products:

  • SeqCap EZ Exome + UTR Kit, 4 Reactions (Catalog No. 06740294001)
  • SeqCap EZ Exome + UTR Kit, 48 Reactions (Catalog No. 06740308001)

Download Design Files

Overview

The SeqCap EZ Exome + UTR Enrichment Kit product is based on the same coding exon sources from SeqCap EZ Human Exome Kit v3.0 with expanded coverage of 5’- and 3’-untranslated regions (UTRs) from the following sources:
NCBI Reference Sequence (RefSeq) refGene table from UCSC GRCh37/hg19 March 2012
Ensembl (GRCh37 v64)

File Descriptions

BED files are plain text, tab-delimited files which list genomic coordinates. These files may be used to explore design targets and to assess capture performance within the targeted regions. The first 3 columns are chromosome or sequence name, target start (0-based), and target end (1-based). For selected SeqCap EZ designs, the fourth column of primary and capture target BED files shows associated gene annotation for each genomic region.

BED files can be imported into Microsoft Excel as tab-delimited text or visualized using the Roche NimbleGen SignalMap software.

The following files are included in the downloadable zip file:

  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_primary_annotated.bed: This file contains the design primary target (unpadded) in hg19 coordinates and gene annotation in the 4th column.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_capture_annotated.bed: This file contains coordinates showing the probe footprint with no padding in hg19 coordinates.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_UCSCBrowser.bed: This file is for viewing target coordinates in the UCSC Genome Browser.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_coverage_summary: This file describes the primary target size, the percentage of primary target bases directly covered by one or probes (“Probe_Coverage”), and the percentage of primary target bases with estimated sequencing coverage for typical results (“Estimated_Coverage”).
     

Download Design Files

Demo Data

Demo data is available for evaluation. Please contact your country Roche representative

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