Target Enrichment Selector

Select the Right Kit for your Target Enrichment Needs - Kapa Biosystems

Select the Right Kit for your Target Enrichment Needs

What are you attempting to enrich before NGS?

  • DNA
  • Bisulfite-treated DNA
  • RNA

Which types of mutations do you want to detect?

  • SNVs only
  • SNVs, indels, CNVs, fusions, etc.

Do you have >100 ng human DNA (and not from FFPE or cfDNA) for library prep?

  • Yes
  • No

Do you want the faster amplicon-based workflow?

  • Yes
  • No

Do you want to enrich human or non-human DNA?

  • Non-human (or mixed genome)
  • Human

Do you want to enrich the exome, exome plus more regions, or custom regions?

  • Custom Regions
  • Exome or more

How large are your target regions?

  • >7-200 Mb
  • 0-7 Mb

Is your focus on analyzing medically relevant regions or discovering novel variants?

  • Analyzing medically relevant
  • Variant discovery

Are you enriching human or non-human DNA?

  • Non-human targets up to 210 Mb
  • Human

Which best describes your goal?

  • Enriching a pre-set panel of targets containing 5.5 million CpGs for an initial broad screen
  • Enriching custom regions that I will specify

How large are your target regions?

  • >90-210 Mb
  • 0-90 Mb

Are you enriching human or non-humanRNA?

  • Non-human targets up to 200 Mb
  • Human

Which best describes your goal?

  • Enriching a pre-set panel of targets containing 17 Mb of long non-coding RNAs
  • Enriching custom regions that I will specify

How large are your target regions?

  • >7-100 Mb
  • 0-7 Mb

HEAT-Seq Probes

HEAT-Seq Enrichment Systems provide an amplification-based enrichment method that combines a fast and easy workflow with powerful SNV detection for targeted resequencing applications in human genetic conditions and cancer research. Employing an advanced version of Molecular Inversion Probe (MIP) technology, the HEAT-Seq Systems are available in catalog and custom formats, delivering reliable performance to enable researchers to get the most out of their time and resources.

  • The HEAT-Seq Oncology Panel targets the coding region of 60 common oncogenes. Ideal for new variant discovery and validation, this panel contains 5,144 probes, and has a capture target of 245 Kb.
  • The HEAT-Seq Ultra Oncology HotSpot Panel is designed for the detection of variants in commonly targeted oncogenic hot spots. Containing 635 probes and with a capture target of 30.5 Kb, this panel is highly scalable and well suited to high-throughput applications where detection of low-frequency variants is critical.
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SeqCap EZ Prime Developer Probes

SeqCap EZ Prime Developer Probes enable enrichment of customer regions of interest up to 200 Mb for non-human genomes and unconventional human designs.

  • Utilize our hybrid capture technology for the enrichment of non-human genomes, powered by our robust design algorithms, for efficient and uniform capture.
  • Simplify your bioinformatics pipeline with the experts you trust. Our expertise spans every taxonomic kingdom; let us create your custom design, with our experience from over 100 species
  • Maximize variant discovery while minimizing sequencing costs. Sequence more samples with your capacity using our efficient capture methods and multiplexing capabilities
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SeqCap EZ Prime Choice XL Probes

SeqCap EZ Prime Choice Library provides researchers with an easily automated, multiplex friendly tool to prepare samples for human targeted resequencing studies. Create your custom SeqCap EZ Prime Choice Design to discover more variants that matters in your research. SeqCap EZ Prime Choice Probes enable enrichment of custom regions of interest from 7 Mb to 200 Mb.

  • Achieve uniform capture, and save on sequencing costs. The NimbleGen Sequence Capture design algorithm utilizes up to
  • 2.1 million probes and ensures high-performance capture.
  • Capture up to 200 Mb custom regions with a single design.
  • Identify more mutations. More complete coverage of target regions with advanced repeat masking methods.
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SeqCap EZ Prime Choice Probes

SeqCap EZ Prime Choice Library provides researchers with an easily automated, multiplex friendly tool to prepare samples for human targeted resequencing studies. Create your custom SeqCap EZ Prime Choice Design to discover more variants that matter in your research. SeqCap EZ Prime Choice Probes enable enrichment of custom regions of interest up to 7 Mb.

  • Achieve uniform capture, and save on sequencing costs. The Roche Sequence Capture design algorithm utilizes up to 2.1 million overlapping probes, allowing capture of up to 7 Mb of custom regions within a single design.
  • Identify more variants. More complete coverage of target regions with advanced repeat masking methods.
  • Optimize throughput and sequencing coverage with sample multiplexing.
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SeqCap EZ MedExome Probes

SeqCap EZ MedExome Probes is a new exome solution that queries the entire human exome with enhanced coverage for exons of disease-associated gene regions. The design targets the genome assembly GRCh38/hg38 (coordinates for hg19 annotation are also available) with comprehensive sequencing coverage of medically research relevant regions and protein coding regions. The product has been extensively optimized in design, empirical rebalancing, and manufacturing to increase coverage in hard-to-sequence regions for a more uniform and complete exome. The SeqCap EZ MedExome product produces greater than 85% on-target rate, demonstrates high uniformity across the targeted region with ~98% sensitivity for SNP detection and >99% specificity for SNP allele classification. Additionally, the SeqCap EZ MedExome product is compatible with a mitochondria-specific design which enables extended exome testing in cases where mitochondrial etiologies may be implicated. In the SeqCap EZ MedExome PLUS configuration, the product offers the ability to add up to 200 Mb of user-defined custom regions to the existing Medexome design.

  • Focus on genomic regions that really matter
    • Utilize a whole exome solution with exceptional coverage of genes from CCDS, RefSeq CDS, Ensembl, GENCODE, miRBase, VEGA and OMIM and maximal coverage for regions of medical research relevance
    • Add up to 200Mb of user-defined content with the SeqCap EZ MedExome PLUS configuration
  • Gain greater efficiency at minimal cost
    • Reduce sequencing cost with fully validated and supported multiplexing protocols
    • Minimize retesting and follow-up sequencing with uniform sequence coverage
  • Call variants with confidence 
    • Obtain greater than 98% bases covered at >20X depth for disease-associated genes.
    • Detect SNPs with 98% sensitivity and 99% specificity for SNP allele classification*

SeqCap EZ Human Exome Probes v3.0

SeqCap EZ Human Exome Probes v3.0 covers more than 20,000 genes in the human genome. All the genome coordinates are based on human genome build GRCh37 (hg19). More than two million oligonucleotide DNA probes capture the target regions. Because the flanking regions of some coding exons and miRNAs are also covered by probes, the total size of the regions covered by probes is 64 Mb.

  • Achieve the most comprehensive coverage of coding regions: Coverage of genes from RefSeq RefGene CDS, CCDS, and miRBase v14 databases, plus coverage of 97% Vega, 97% Gencode, and 99% Ensembl databases.
  • Experience the highest target enrichment efficiency: The Roche Sequence Capture advanced design algorithm coupled with 2.1 million long oligonucleotide probes delivers superior target enrichment performance to discover more variants, including both single nucleotide variants and indels.
  • Maximize variant discovery while minimizing sequencing costs: Reduce the sequencing output needed to discover and/or validate deleterious mutations.
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SeqCap Epi Developer Probes

SeqCap Epi Developer Probes, as part of the SeqCap Epi Enrichment System, enables the targeting of selected genomic regions from bisulfite treated genomic DNA in a single workflow for your genome of interest.

  • Discover differential methylation efficiently: Target your region of interest, up to 210Mb, in the epigenome with the only custom enrichment solution for bisulfite sequencing, delivering better depth of coverage1 and higher resolution than Whole Genome Bisulfite Sequencing.
  • Optimized design and workflow to effectively conserve epigenome complexity: The SeqCap Epi System’s advanced design algorithm coupled with the ability to manufacture millions of long oligonucleotide probes delivers superior2 target enrichment performance, which enables you to observe methylation changes on both strands while preserving the ability to decipher between SNPs vs. methylation events, with an industry leading sample input of just 1ug.
  • Maximize epigenetic discovery while minimizing sequencing costs: Reduce the sequencing output needed to study methylation landscapes by starting with your regions of interest.

1. Allum F, Shao X, Guénard F, et al. Characterization of functional methylomes by next-generation capture sequencing identifies novel disease-associated variants. Nat Commun. 2015 May 29;6:7211.

2. L Q, Suzuki M, Wendt J, et al. Post-conversion targeted capture of modified cytosines in mammalian and plant genomes. Nucleic Acids Res. 2015 Jul 13;43(12):e81.

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SeqCap Epi CpGiant Probes

SeqCap Epi CpGiant Probes is a fixed epigenome-wide design, that allows researchers to interrogate >5.5 million methylation sites per sample at single-nucleotide resolution.

  • Upgrade your research from microarrays to sequencing: Get the whole picture by interrogating more than 5.5 million CpGs compared to the 450K CpGs on the Illumina Infinium HumanMethylation450 BeadChip while enjoying the cost benefits of targeted bisulfite sequencing compared to Whole Genome Bisulfite Sequencing (WGBS).
  • Optimized design and workflow to effectively conserve epigenome complexity: The SeqCap Epi System’s advanced design algorithm coupled with the ability to manufacture millions of long oligonucleotide probes delivers superior1 target enrichment performance, which enables you to observe methylation changes on both strands while preserving the ability to decipher between SNPs vs. methylation events, with an industry leading sample input of just 1ug.
  • Maximize epigenetic discovery while minimizing sequencing costs: Reduce the sequencing output needed to study methylation landscapes by starting with the best studied2 CpG islands in the human genome.

1. L Q, Suzuki M, Wendt J, et al. Post-conversion targeted capture of modified cytosines in mammalian and plant genomes. Nucleic Acids Res. 2015 Jul 13;43(12):e81.

2. Allum F, Shao X, Guénard F, et al. Characterization of functional methylomes by next-generation capture sequencing identifies novel disease-associated variants. Nat Commun. 2015 May 29;6:7211.

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SeqCap Epi Choice Probes

SeqCap Epi CpGiant Probes is a fixed epigenome-wide design, that allows researchers to interrogate >5.5 million methylation sites per sample at single-nucleotide resolution.

  • Upgrade your research from microarrays to sequencing: Get the whole picture by interrogating more than 5.5 million CpGs compared to the 450K CpGs on the Illumina Infinium HumanMethylation450 BeadChip while enjoying the cost benefits of targeted bisulfite sequencing compared to Whole Genome Bisulfite Sequencing (WGBS).
  • Optimized design and workflow to effectively conserve epigenome complexity: The SeqCap Epi System’s advanced design algorithm coupled with the ability to manufacture millions of long oligonucleotide probes delivers superior1 target enrichment performance, which enables you to observe methylation changes on both strands while preserving the ability to decipher between SNPs vs. methylation events, with an industry leading sample input of just 1ug.
  • Maximize epigenetic discovery while minimizing sequencing costs: Reduce the sequencing output needed to study methylation landscapes by starting with your regions of interest.

1. L Q, Suzuki M, Wendt J, et al. Post-conversion targeted capture of modified cytosines in mammalian and plant genomes. Nucleic Acids Res. 2015 Jul 13;43(12):e81.

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SeqCap RNA Developer Probes

SeqCap RNA Developer Probes, as part of the SeqCap RNA Enrichment System, are a custom solution that enables the targeted capture in non-human organisms or non-conventional enrichment against the genes or transcripts of interest. These probes are customizable probe pools that can target coding and non-coding transcripts, with up to 200 Mb design size. Theys come in 12, 48 and 384 reaction sizes. Each reaction can be used for multiple samples through pre-capture multiplexing.

  • Utilize industry-leading expertise in less well annotated genomes
  • Discover with More Confidence: Robust detection of rare transcripts with efficiently conserved transcript abundance using our high sensitivity RNA hybrid capture
  • Broaden Your Research Scope: Maximize the discovery and characterization of novel exons, splicing variants, mutations and gene fusions through the ability to target your transcripts of interest with up to 200Mb custom designs
  • Experience Superior Efficiency1: Increase your sample capacity with the same amount of sequencing while utilizing a greatly simplified workflow that enables pre-capture multiplexing

1. Clark MB, Mercer TR, Bussotti G, et al. Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing. Nat Methods. 2015 Apr;12(4):339-42.

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SeqCap lncRNA Probes

SeqCap lncRNA Probes, as part of the SeqCap RNA Enrichment System, enables the targeting of lncRNAs (Long non-coding RNAs). These non-protein coding transcripts, longer than 200 nucleotides, are quite abundant. LncRNA has been reported1 associated with various diseases or biological processes. These probes come in 4, 48 and 384 reaction sizes. Each reaction can be used for multiple samples through pre-capture multiplexing.

  • Selectively target lncRNAs, a common, but not well understood, component of the human transcriptome
  • Achieve unprecedented depth and sensitivity1 for rare transcript detection with effectively conserved transcript abundance
  • Maximize the discovery and characterization of transcript variants and isoforms
  • Investigate the developmental regulation of lncRNAs
  • Investigate the tissue- and cell-type specificity of lncRNAs
  • Investigate the role of lncRNA in disease

1. Bussotti G, Leonardi T, Clark M, et al. Improved definition of the mouse transcriptome via targeted RNaA sequencing. Genome Research. 2016. 26;705-716


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SeqCap RNA Choice XL Probes

SeqCap RNA Choice Probes are customizable probe pools that can target coding and non-coding transcripts. They are designed for less than 7Mb of target size; SeqCap RNA Choice XL Probes are designed for less than 7-100 Mb of target size. Both probes are available in 12, 48, and 384 reaction probes allowing for support of projects with small and large sample numbers. Each reaction can be used for multiple samples through pre-capture multiplexing.

  • Discover with More Confidence: Achieve unprecedented1 depth and sensitivity for rare transcript detection with effectively conserved transcript abundance
  • Broaden Your Research Scope: Maximize the discovery and characterization of novel exons, splicing variants, mutations and gene fusions through the ability to target your transcripts of interest with up to 200Mb custom designs
  • Experience Superior Efficiency: Increase1 your sample capacity with the same amount of sequencing while utlizing a greatly simplified workflow that enables pre-capture multiplexing
  • Minimize input material requirements: Strand specific cDNA library is constructed from 10 ng – 1ug of either poly(A)-selected, ribosomally-depleted, or total RNA.

1. Bussotti G, Leonardi T, Clark M, et al. Improved definition of the mouse transcriptome via targeted RNaA sequencing. Genome Research. 2016. 26;705-716


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SeqCap RNA Choice Probes

SeqCap RNA Choice Probes are customizable probe pools that can target coding and non-coding transcripts. They are designed for less than 7Mb of target size, SeqCap RNA Choice XL Probes are designed for less than 7-100 Mb of target size. Both probes are available in 12, 48, and 384 reaction probes allowing for support of projects with small and large sample numbers. Each reaction can be used for multiple samples through pre-capture multiplexing.

  • Discover with More Confidence: Achieve greater depth and sensitivity for rare transcript detection with effectively conserved transcript abundance
  • Broaden Your Research Scope: Maximize the discovery and characterization of novel exons, splicing variants, mutations and gene fusions through the ability to target your transcripts of interest with up to 200Mb custom designs
  • Experience Superior Efficiency1: Increase your sample capacity with the same amount of sequencing while utilizing a greatly simplified workflow that enables pre-capture multiplexing
  • Minimize input material requirements: Strand specific cDNA library is constructed from 10 ng – 1ug of either poly(A)-selected, ribosomally-depleted, or total RNA.

1. Clark MB, Mercer TR, Bussotti G, et al. Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing. Nat Methods. 2015 Apr;12(4):339-42.

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