The SeqCap® EZ HE-Oligo Kit B is used to block library adapters in hybridization to prevent secondary capture. The kit is intended for customers performing sequence capture and sequencing using Illumina systems.
The kit includes hybridization enhancing (HE) oligos for barcoding 1 to 12 samples corresponding to Illumina’s TruSeq Library Kit B; each HE oligo can be used for 8 separate reactions. It is intended for barcoding 13 or more samples in multiplex experiments when SeqCap EZ HE-Oligo Kit A has been used to barcode samples 1 to 12.
To ensure proper matches during the hybridization step, the DNA Adapter Index from the Adapter Kit B corresponds to the HE Oligo from this kit.