Reagents and Accessories

Overview

Target enrichment, where specific regions of interest are enriched, is a more cost-effective next-generation sequencing (NGS) technique compared to whole genome sequencing. Roche Sequencing Solutions offers high-quality reagents and consumables suitable for enrichment by either amplicon or hybrid capture strategies.

Features and Benefits

SeqCap® Accessory Kit V2

  • COT Human DNA and KAPA HiFi HotStart ReadyMix to perform sequence capture experiments using SeqCap EZ probe panels
  • Blocks repetitive regions during hybridization and enables ligation-mediated PCR (LM-PCR)
     

SeqCap Adapter Kit A

SeqCap Adapter Kit B

  • DNA library construction using KAPA Library Preparation Kits and KAPA Stranded RNA-Seq Library Preparation Kits
  • Facilitates precapture LM-PCR and sequencing on Illumina platforms
     

SeqCap Pure Capture Bead Kit

  • SeqCap Beads for recovery of captured DNA after hybridization and AMPure XP Beads to purify amplified LM-PCR products
  • Recovery of captured DNA and purification of amplified LM-PCR products
     

SeqCap Hybridization and Wash Kit

  • Reagents for hybridization and wash steps to perform sequence capture experiments using SeqCap EZ probe panels
  • Hybridization of probes to DNA libraries and washing of hybridized libraries
     

SeqCap Oligo Kit A

SeqCap Oligo Kit B

  • Oligonucleotides to block library adapters in hybridization
  • Prevention of secondary capture
     

SeqCap EZ Developer Reagent

  • Reagent to block repetitive regions in the genome when Cot1 DNA is not available
  • Blocks repetitive regions during hybridization