SeqCap EZ Exome + UTR Kit

Overview

The SeqCap® EZ Human Exome + UTR Kit enables enrichment of 64 Mb of coding exons and miRNA regions, plus 32 Mb of untranslated regions (UTRs). The kit is based on the same coding exon sources from SeqCap EZ Human Exome Kit v3.0 with expanded coverage of 5'- and 3' UTRs. Information for UTRs was obtained from NCBI Reference Sequence (RefSeq) refGene table from UCSC GRCh37/hg19 March 2012 and Ensembl (GRCh37 v64).

Benefits of the SeqCap EZ Human Exome + UTR Kit

  • A comprehensive exome and UTR enrichment solution
  • Minimized sequencing costs with the superior capture efficiency of our 2.1M overlapping probes
  • Ability to discover more variants compared to all other target enrichment solutions*

Design Files

Design and Annotation files were designed for use with the following products:

  • SeqCap EZ Exome + UTR Kit, 4 Reactions (Catalog No. 06740294001)
  • SeqCap EZ Exome + UTR Kit, 48 Reactions (Catalog No. 06740308001)

Download Design Files 

 

File Descriptions

BED files are plain text, tab-delimited files which list genomic coordinates. These files may be used to explore design targets and to assess capture performance within the targeted regions. The first 3 columns are chromosome or sequence name, target start (0-based), and target end (1-based). For selected SeqCap EZ designs, the fourth column of primary and capture target BED files shows associated gene annotation for each genomic region.

BED files can be imported into Microsoft Excel as tab-delimited text or visualized using the SignalMap software.

The following files are included in the downloadable zip file:

  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_primary_annotated.bed: This file contains the design primary target (unpadded) in hg19 coordinates and gene annotation in the 4th column.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_capture_annotated.bed: This file contains coordinates showing the probe footprint with no padding in hg19 coordinates.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_UCSCBrowser.bed: This file is for viewing target coordinates in the UCSC Genome Browser.
  • SeqCap_EZ_ExomeV3_Plus_UTR_hg19_coverage_summary: This file describes the primary target size, the percentage of primary target bases directly covered by one or more probes (“Probe_Coverage”), and the percentage of primary target bases with estimated sequencing coverage for typical results (“Estimated_Coverage”).

Demo Data

Demo data is available for evaluation. Please contact Roche representative for your country. 

*Data on file.

Product Catalog Number
Pack Size How to Buy
SeqCap EZ Exome+UTR
0674029400
4 Reactions
Contact Us
SeqCap EZ Exome+UTR
06740308001
48 Reactions
Contact Us

Reagents and accessories are available separately.