Overview

KAPA SYBR FAST qPCR Kits contain the first DNA polymerase engineered via our directed evolution technology to be more tolerant of SYBR Green I dye inhibition. The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis compared to traditional kits.* 

Benefits of KAPA SYBR FAST qPCR Kits

  • Optimal performance in stringent real-time PCR reaction conditions
  • Improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity compared to other commercial kits*


Refer to Instrument Compatibility Chart for guidance on compatible platforms. 

 

Product Highlights

Quantitate changes in gene expression more accurately

  • High reaction efficiency between 95 – 105%, improving accuracy and reproducibility
  • Unbiased efficiency across a wide range of GC contents and amplicon lengths

Detect low copy and difficult targets consistently

  • Improved processivity results in earlier Cq scores
  • Higher fluorescence detection across varying AT- and GC-rich targets

Complete real-time PCR runs in just 40 minutes

  • 55% shorter run times with fast cycling protocol
  • High performance when switching from standard to fast protocols

*Data on file.