KAPA HRM FAST qPCR Kits

Overview

High Resolution Melt (HRM) analysis is a technique for fast, high-throughput post-PCR analysis of genetic mutations or variance in nucleic acid sequences. The KAPA HRM FAST Master Mix contains a novel DNA polymerase and buffer system, and the EvaGreen saturating dye optimized for maximum discrimination between sequence variants.

Benefits of HRM FAST qPCR Kit

  •  Accurate detection of DNA sequence variants

Product Highlights

Maximized differences in sequence variants

  • Contains DNA polymerase engineered to maximize differences in melting behavior
  • Improved sensitivity

Accurate detection of Type IV SNPs

  • 95% detection levels compared to a competitor kit*
  • Reduced reaction times

Extraction, amplification and HRM in < 2 hours

  • Rapid detection of SNPs when used in combination with the KAPA Express Extract Kit

*Data on file.

Kits can be stored for up to 12 months at -20˚C.

Kits include KAPA HRM FAST qPCR Master Mix (2X), which contains KAPA HRM FAST DNA Polymerase, reaction buffer, dNTPs and EvaGreen saturating dye. MgCl2 (25 mM) is supplied separately.

 

Licensing Information

Certain applications of this product are covered by patents issued to parties other than Kapa Biosystems and applicable in certain countries. Purchase of this product does not include a license to perform any such applications. Users of this product may therefore be required to obtain a patent license depending upon the particular application and country in which the product is used.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056, 6,171,785, and 5,928,907 (claim numbers 12-24, 27-28). The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Kapa Biosystems Inc. is licensed by Biotium Inc. to sell reagents containing EvaGreen dye for use in real-time PCR, for research purposes only.

Licensed under U.S. Patent nos. 5,338,671 and 5,587,287 and corresponding patents in other countries.

The purchase of this product includes a limited, non-transferable license under specific claims of U.S. Patent Nos. 6,174,670, 6,569,627 and 5,871,908, owned by the University of Utah Research Foundation or Evotec Biosystems GmbH and licensed to Idaho Technology, Inc. and Roche Diagnostics GmbH, to use only the enclosed amount of product according to the specified protocols. No right is conveyed, expressly, by implication, or by estoppel, to use any instrument or system under any claim of U.S. Patent Nos. 6,174,670, 6,569,627 and 5,871,908, other than for the amount of product contained herein.

EvaGreen is a registered trademark of Biotium, Inc.

Rotor-Gene is a trademark of Corbett Research.

Specifications
Spec
Description
Starting Material
DNA, cDNA
Input Amount
100 pg - 20 ng
Components
Component
2X KAPA HRM FAST Master Mix
MgCl2 (25 mM)

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Kits include KAPA HRM FAST qPCR Master Mix (2X), which contains KAPA HRM FAST DNA Polymerase, reaction buffer, dNTPs and EvaGreen saturating dye. MgCl2 (25 mM) is supplied separately.

Kit Code
Roche Cat. No
Description
Kit Size
How to buy
KK4201
07959290001
100 x 20 µL reactions 1 mL
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KK4202
07959303001
500 x 20 µL reactions
5 mL
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KK4203
07959311001
1000 x 20 µL reactions
10 mL
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