qPCR

Overview

Quantitative real-time PCR (qPCR) enables the detection and quantification of PCR products in real time and is used widely in gene expression studies due to its high sensitivity, reproducibility and wide dynamic range. The technique uses double-stranded DNA (dsDNA)-binding dyes or target-specific fluorescently labeled primers and probes that hybridize with the target sequence to measure the relative amount of DNA. Most commercially available qPCR reagents contain wild-type, unmodified DNA polymerase, but these enzymes were never intended as tools for use in molecular biology.  The performance differences among commercial qPCR kits are the result of differences in factors like buffer formulation and/or enzyme concentration.

Roche offers KAPA qPCR reagents containing DNA polymerase and other novel enzymes specifically selected through our directed evolution technology for qPCR applications. These confer significant improvements to reaction efficiency, sensitivity, speed, and signal-to-noise ratio.

Gene Expression

KAPA SYBR FAST qPCR Kits

KAPA SYBR FAST One-Step qRT-PCR Kits

KAPA PROBE FAST qPCR Kits

KAPA PROBE FORCE qPCR Kits

  • Higher sensitivity and improved reaction efficiency across a broad range of amplicon sizes and GC contents*
  • Reduced experimental variation and contamination with a convenient one-step qRT-PCR protocol*
  • Optimized for versatility and speed, providing highly reproducible results for gene expression assays
  • Direct qPCR from crude blood, tissue, and plant extracts.
  • Sample-to-Cq (workflows in <1 hour*)
  • High efficiency for accurate, reproducible, and sensitive results
Genotyping

KAPA SYBR FAST qPCR Kits

KAPA PROBE FAST qPCR Kits

KAPA HRM FAST qPCR Kits

KAPA PROBE FORCE qPCR Kits

  • Higher sensitivity and improved reaction efficiency across a broad range of amplicon sizes and GC contents*
  • Optimized for versatility and speed, compatible with all probe-based SNP detection applications
  • Engineered to maximize differences in melting behavior between sequence variants resulting in accurate SNP genotyping with maximum sensitivity and speed
  • Direct qPCR from crude blood, tissue, and plant extracts.
  • Sample-to-Cq (workflows in <1 hour)
  • High efficiency for accurate, reproducible, and sensitive results
Food and Water Safety

KAPA PROBE FORCE qPCR

Kits

  • Direct qPCR from crude blood, tissue, and plant extracts
  • Sample-to-Cq (workflows in <1 hour)
  • High efficiency for accurate, reproducible, and sensitive results

KAPA SYBR FAST qPCR Kits

Containing the first DNA polymerase engineered via directed evolution technology to be more tolerant to SYBR Green I dye inhibition, KAPA SYBR FAST qPCR Kits confer high sensitivity and reaction efficiency across a broad range of amplicon sizes and GC contents.

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KAPA SYBR FAST One-Step qRT-PCR Kits

Designed for optimal performance in stringent qRT-PCR reaction conditions, KAPA SYBR FAST One-Step qRT-PCR Kits are optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination.

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KAPA PROBE FORCE qPCR Kits

Containing a highly inhibitor-resistant qPCR master mix with a third-generation DNA polymerase selected through our direct evolution technology, the KAPA PROBE FORCE qPCR Kits remove the need for DNA purification and enable streamlined sample-to-Cq workflows.

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KAPA PROBE FAST qPCR Kits

Optimized for versatility and speed, and compatible with all probe-based SNP detection applications, KAPA PROBE FAST qPCR Kits provide fast and reproducible results for qPCR experiments using sequence-specific fluorogenic probe chemistries.

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KAPA HRM FAST qPCR Kits

Engineered to maximize differences in melting behavior between sequence variants, KAPA HRM FAST qPCR Kits yield accurate SNP genotyping with high sensitivity and speed.

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*Data on file.