KAPA Adapters are recommended for use with KAPA Stranded RNA-Seq Kits with RiboErase. However, kits are also compatible with non-indexed, single-indexed, and dual-indexed adapters that are routinely used in Illumina TruSeq, Roche NimbleGen SeqCap EZ, Agilent SureSelect, and other similar library construction and target capture workflows. Custom adapters that are of similar design and are compatible with “TA-ligation” of dsDNA may also be used, remembering that custom adapter designs may impact library construction efficiency.
While it is not necessary to adjust adapter concentrations to accommodate moderate sample-to-sample variation, an adapter concentration appropriate for the amount of input RNA is recommended. The table below summarizes recommended adapter concentrations for various inputs into the rRNA depletion reaction.
|Quantity of starting material
||Adapter stock concentration
||Adapter Concentration in ligation reaction
Low Concentration (1.5 µM) KAPA Adapter Kits are recommended for all inputs. For assistance with adapter compatibility and ordering, please visit kapabiosystems.com/support.