Library preparation for next generation sequencing (NGS) involves several steps – fragmentation of nucleic acids to required lengths, end repair and ligation of adapters to ends of target sequences, library amplification and quantification. A critical step in DNA and RNA library construction is to select for DNA fragments of appropriate size and to remove adapter dimers. Adapter quality and bead performance can have a profound impact on the outcome of library construction, particularly when working with low-input and challenging samples.
Roche Sequencing Solutions offers high-quality KAPA Pure Beads and KAPA Adapters that are functionally tested for NGS library construction. They are supplied as stand-alone products to support flexibility.