Library Amplification

Overview

Library preparation for next-generation sequencing (NGS) often involves an amplification step for obtaining fragments enriched for adapter ligated ends and for increasing the library size, both of which are essential for effective detection of sequences during a sequencing run. However, PCR can often introduce bias for sample composition, leading to potential underrepresentation of these fragments in a sequencing read.

Roche Sequencing Solutions offers KAPA Library Amplification Kits, which contain KAPA HiFi DNA polymerase, a novel enzyme engineered through our directed evolution technology, for ultra-high fidelity and robustness. KAPA HiFi improves the overall sequencing quality with low amplification bias, more uniform coverage of difficult regions, lower duplication rates compared to other options, and amplifies NGS libraries with industry-leading fidelity.*

Benefits of Library Amplification Kits

  •  Efficient amplification of GC- and AT-rich fragments and  genome
  •  Low amplification bias and high sequencing coverage
  •  Industry-leading fidelity*
  •  Efficient amplification of uracil-containing templates. 

Roche Sequencing Solutions offers library amplification kits for high-quality NGS library preparations using normal and bisulfite-converted DNA.

 

KAPA Library Amplification Kits

Containing KAPA HiFi Polymerase, considered the gold standard for library preparation, the KAPA Library Amplification Kits provide high overall sequence quality, uniform coverage of GC- and AT-rich regions, and low amplification bias.

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KAPA HiFi Uracil+ Kits

As the uracil-tolerant product of the KAPA Library Amplification Kit portfolio, KAPA HiFi Uracil+ Kits offer the same improvements enabled by the KAPA HiFi Polymerase to bisulfite-converted DNA.

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*Data on file.