KAPA Dual- or Single-Indexed Adapters are recommended for use with KAPA Library Preparation Kits, except for methyl-seq applications. KAPA Library Preparation Kits are also compatible with non-indexed, single-indexed, and dual-indexed adapters that are routinely used in SeqCap EZ, Illumina TruSeq, Agilent SureSelect, and other similar library construction and target capture workflows. Custom adapters that are of similar design and are compatible with “TA-ligation” of dsDNA may also be used, remembering that custom adapter designs may impact library construction efficiency.
Ligation efficiency is robust for adapter-insert molar ratios from 10:1 to 50:1. As a general guideline, an adapter-insert molar ratio of ~10:1 is recommended for libraries constructed from ≥100 ng of fragmented DNA. Higher adapter-insert molar ratios may be beneficial for low-input and challenging samples.
High Concentration (30 µM) KAPA Single-Indexed Adapter Kits are recommended for library construction from from ≥100 µg inputs, whereas Low Concentration (1.5 µM) KAPA Single-Indexed Adapter Kits are recommended for inputs ≤100 ng. Around 100 ng, the most appropriate concentration depends on library insert size. KAPA Dual-Indexed Adapters may be used for all inputs with the appropriate dilution. For assistance with adapter compatibility and ordering, please visit kapabiosystems.com/support.