DNA Library Preparation

Overview

Preparation of a DNA library for next generation sequencing (NGS) platforms entails several steps that have a direct influence on the quality of the sequencing library, and ultimately on the reliability of sequencing results.  Roche Sequencing Solutions offers best-in-class* DNA library preparation kits containing high-quality enzymes and reaction buffers developed through our Directed Evolution Technology. The KAPA Library Preparation Kits include modules required for end repair, A-tailing, ligation, and amplification (for Illumina platforms) and offer complete library preparation solutions with KAPA Adapters and KAPA Pure Beads.

Benefits of the KAPA Library Preparation Kits

• Compatibility with a wide variety of DNA inputs and sample types and high yields of adapter-ligated library molecules 

• Reduced PCR amplification bias resulting in improved sequencing coverage compared to other options*

• Kits without an amplification module for PCR-free workflows

• Ultra-pure, high-quality library construction reagents supplied in convenient master-mix formats

• Competitive per library cost

Roche Sequencing Solutions offers three generations of KAPA Library Preparation Kits: HTP/LTP, HyperPrep and HyperPlus, for library construction on the Illumina platform.

 

Featured Webinar

Challenges of Library Construction Part 1 
Presenter: Dr. Maryke Appel, Roche

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KAPA HyperPlus Kits

As the latest and the third-generation option, KAPA HyperPlus Kits provide a streamlined workflow with integrated, low-bias enzymatic fragmentation and library preparation in a single tube, offering industry-leading library yields* and superior sequencing results. Kits are compatible with the Illumina sequencing platform.

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KAPA HyperPrep Kits

As the second-generation option, KAPA HyperPrep Kits provide a novel one-tube chemistry and a streamlined workflow, offering superior library yields* and sequencing results from Covaris-sheared DNA.

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KAPA HTP/LTP Library Preparation Kits

KAPA HTP/LTP Library Preparation Kits are the first generation products providing high performance with a three-step “with bead” protocol. Kits are compatible with the Illumina sequencing platform.

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*Data on file.