Library Preparation

Overview

Preparing target nucleic acids for next-generation sequencing

Library construction is the first and a critical step in the next generation sequencing (NGS) workflow. DNA or RNA samples are first fragmented to the appropriate size and then sequencing platform-specific adapters are added. In most cases, libraries have to be amplified before the next step in the process (target enrichment or sequencing). Each step can potentially introduce errors or biases that could significantly impact library quality and ultimately affect the quality and reliability of sequencing results. It is therefore imperative to use the highest quality reagents and optimized protocols to obtain the best sequencing libraries, lowest bias, and the highest and most uniform coverage.

Roche Sequencing Solutions offers a range of library preparation kits that delivers the highest quality sequencing libraries suitable for use in different sequencing platforms. Kits contain high-quality enzymes selected through our Directed Evolution Technology, and formulated in convenient, easy-to-use master mixes. 

...

Library Quantification

Quantify NGS libraries reliably and accurately with DNA standards and qPCR primers produced with stringent quality control. Amplify diverse DNA fragments with comparable efficiency and maximum lot-to-lot consistency using the KAPA Library Quantification Kits containing KAPA SYBR FAST DNA Polymerase engineered using our directed evolution technology.

Learn more
...

DNA Library Preparation

Utilize three generations of KAPA DNA Library Preparation Kits for library construction on the Illumina platform with minimal fragmentation and amplification bias. Select from options with single tube chemistry or a three-step “with bead” protocol and get exceptional library yield and diversity, and the ability to use a wide variety of samples and automate protocols.

Learn more
...

Library Amplification

Amplify your NGS library using the KAPA Library Amplification Kits containing KAPA HiFi DNA Polymerase – the gold standard for NGS library amplification – selected through our directed evolution technology and engineered specifically for ultra-high fidelity, high efficiency, low bias and robustness.*

Learn more
...

RNA Library Preparation

Select from two generations of KAPA RNA library preparation kits for RNA library construction on the Illumina platform to get improved detection of GC-rich, low-abundance or degraded (FFPE) transcripts. Construct your RNA library in a single workday with options for enhancing specifically for mRNA, depleting rRNA or with no upfront enrichment of RNA.

Learn more
...

Library Preparation Accessories

Get complete DNA and RNA library preparation solutions by incorporating KAPA Adapters and KAPA Pure Beads in your workflow. Achieve efficient library construction and negligible levels of barcode cross contamination with functionally tested KAPA Adapters and obtain tunable size selection parameters and efficient reaction cleanups with KAPA Pure Beads.

Learn more

*Data on file.